Apparatus for preparing bladder acellular matrix through physiological process simulation

Abstract

The invention provides a preparation apparatus of the Bladder Acellular Matrix (BAM) by simulating a physiological process. The apparatus comprises the jars, the silicone tubes, the peristaltic pumps,the T-joints, the perfusion needles, and a bladder-like part with 'ureters' and 'urethras'. After the two perfusion needles are connected to the 'ureter' on both sides of the bladder-like part, the bladder-like part is suspended and fixed. Two jars with decellularized liquid are connected with two silicone tubes, wherein the grooves of the two peristaltic pumps are pressed by the tubes. The otherend of the two silicone tubes are respectively connected with two T-joints, and the needle cap part of the perfusion needles connected to the 'ureters' are respectively connected to the two T-jointsto complete the assembly of the preparation apparatus for the BAM. The BAM preparation by the apparatus is fast, and the cell components of the bladder-like part are removed to the maximum extent while preserving as much as possible the composition and structure of the extracellular matrix. The apparatus can effectively simulate the physiological process of the bladder tissue and prepare the BAM quickly and efficiently.

Description

technical field [0001] The invention relates to the technical field of tissue engineering construction, in particular to a preparation device for bladder acellular matrix simulating physiological processes. Background technique [0002] The bladder is a hollow muscular organ whose main function is to store urine at low and stable pressure without causing reflux, while being able to empty urine spontaneously. Currently, bladder tumors are highly prevalent all over the world, especially bladder tumors with muscle invasion often require total cystectomy. In addition, bladder contractures, congenital deformities, trauma, etc., require surgical repair and reconstruction. At present, repairing and reconstructing the bladder using the intestinal tract is still the gold standard for clinically repairing and reconstructing bladder defects. However, the use of intestinal repair to reconstruct the bladder is often accompanied by a series of complications: mucus secretion, long-term ba...

AI Technical Summary

Problems solved by technology

However, mild decellularization reagents mean that it takes a long time for decellularization. If the time is shortened, cell components will remain, which will affect the biocompatibility of the material, which is not conducive to the construction of tissue engineering bladder in the later stage.

If the decellularization solution with strong decellularization performance is used instead, the decellularization time can be shortened, but it may cause the loss of extracellular matrix components, which may affect the adhesion, proliferation, differentiation, etc. of the seed cells, and the outcome may also be tissue engineering bladder. preparation failure

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