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Application of geniposide in alleviating skeletal muscle fibrosis

A technology for geniposide and skeletal muscle, which is applied in the field of natural product therapy, and can solve the problems that geniposide has no reported effect on skeletal muscle fibrosis

Active Publication Date: 2018-08-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, studies have shown that geniposide can alleviate liver fibrosis by inhibiting TGF-β / Smad and ERK-mitogen-activated protein kinase (MAPK) signaling pathways, but so far, geniposide has no effect on skeletal muscle fibrosis There has not been any report on the mitigating effect of

Method used

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  • Application of geniposide in alleviating skeletal muscle fibrosis
  • Application of geniposide in alleviating skeletal muscle fibrosis
  • Application of geniposide in alleviating skeletal muscle fibrosis

Examples

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Embodiment 1

[0026] Example 1 Extraction of geniposide and verification of link skeletal muscle fibrosis

[0027] 1 Experimental method

[0028] 1.1 Preparation method of gardenia meal water extract and alcohol extract

[0029] (1) Preparation of gardenia meal water extract

[0030] Grind the gardenia meal and pass through a 60-mesh sieve. Weigh 15g of gardenia meal powder and add 150mL of deionized water to extract in a water bath at 60°C for 1 hour. After filtering, keep the supernatant, then add 100mL deionized water to the filter residue, and place in a water bath at 60°C for 1h. Similarly, the third leaching is to add 50mL deionized water to the filter residue. After the water bath for 1 h, all the supernatants were mixed, cooled, centrifuged at 4000 rpm for 10 min, and the supernatants were collected. Purification was carried out with macroporous adsorption resin HPD-100A. Firstly carry out pretreatment to HPD-100A macroporous resin: carry out dynamic elution with 95% ethanol, ...

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Abstract

The invention discloses an application of geniposide in alleviating skeletal muscle fibrosis, and belongs to the field of natural product treatment. C2C12 myoblasts are stimulated by the geniposide, and a result indicates that the geniposide can significantly alleviate the fibrosis degree of fibrocytes. Furthermore, by establishing a mice model with contusion-induced skeletal muscle injury, a geniposide standard product is intraperitoneally injected for treating mice, and the geniposide is proved to play an anti-fibrosis role in damaged skeletal muscles. In addition, the fibrosis degree of theC2C12 myoblasts stimulated by TGF-Beta and Smad 4 is increased significantly, but the fibrosis level is decreased significantly after the C2C12 myoblasts are treated with the geniposide as compared with that of untreated C2C12 myoblasts. Therefore, the action mechanism of the geniposide in alleviating skeletal muscle fibrosis can be closely related to a TGF-beta / Smad4 signaling pathway.

Description

technical field [0001] The present invention relates to a new application of geniposide, specifically the application of geniposide in alleviating skeletal muscle fibrosis, and belongs to the field of natural product treatment. Background technique [0002] Skeletal muscle is the organ most directly involved in the movement of the human body, accounting for about 40%-50% of the total weight of the human body. It is also the largest tissue in the human body and plays an important role in human activities, social interactions and occupations. Skeletal muscle injury is a common soft tissue injury in sports medicine, and skeletal muscle is a tissue with complete regenerative capacity. After acute traumatic injury, skeletal muscle can mostly complete regeneration and repair. After injury, skeletal muscle tissue will sequentially go through several processes of inflammation, muscle satellite cell proliferation and activation, and regeneration fiber maturation and remodeling or fib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P21/00A61P29/00A61P17/02A61K8/60A61Q19/00
CPCA61K8/602A61K31/7048A61K36/744A61P17/02A61P21/00A61P29/00A61Q19/00
Inventor 王立潘海鸥李言钱海峰张晖齐希光
Owner JIANGNAN UNIV
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