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Online solid phase extraction liquid chromatography method for detecting clonazepam content in blood

A clonazepam and chromatographic analysis technology is applied in the field of clinical blood drug concentration monitoring of psychotropic drugs, which can solve the problems of long analysis time and complicated pretreatment, and achieves shortening analysis time, reducing toxic and side effects, and reducing personnel operations. Effect

Active Publication Date: 2018-08-24
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology

High-performance liquid chromatography is accurate in quantification, good in reproducibility, and good in economic benefits, but there are still problems such as complicated pretreatment and long analysis time

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  • Online solid phase extraction liquid chromatography method for detecting clonazepam content in blood
  • Online solid phase extraction liquid chromatography method for detecting clonazepam content in blood
  • Online solid phase extraction liquid chromatography method for detecting clonazepam content in blood

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Embodiment Construction

[0035] A kind of online solid-phase extraction liquid chromatography analysis method of clonazepam content in detection blood of the present invention, it comprises the following steps:

[0036] (1) Calibration of standard solution

[0037]First, use a pipette gun to mix 10 μL of standard working solution, 10 μL of standard internal standard solution and 190 μL of blank serum in 1.5ml centrifuge tubes with seven different concentrations to prepare seven standard solutions. After vortex mixing for 30 seconds, add 200 μL of precipitant containing 0.2 mol / L zinc sulfate methanol solution (precipitant is prepared by ultrasonically dissolving 0.2 mol zinc sulfate in 1 L of methanol), and vortex mix at 2000 rpm. After homogenizing for 3 minutes, and then centrifuging at a high speed at 12000 rpm for 5 minutes, the supernatant was obtained, and 200 uL of the above-mentioned supernatant was taken respectively, and the above-mentioned supernatant was detected with a high-performance li...

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Abstract

The invention discloses an online solid phase extraction liquid chromatography method for detecting clonazepam content in blood. The method is to calibrate a standard solution by using high performance liquid chromatograph and a DVD detector, a standard curve equation obtained is y=a*x+b, a blood sample to be tested is taken, after the blood to be tested is processed, the sample to be tested is also detected by using the high performance liquid chromatograph and the DVD detector, a blood y value to be tested is obtained, the blood y value to be tested is substituted into the standard curve equation, the relative concentration x of clonazepam is calculated in the blood sample to be tested, and the concentration of internal standard working fluid is known, thereby calculating the concentration of the clonazepam in the blood to be tested in the sample; the serum of the method is directly injected after sedimentation and centrifugation, which makes the detection process simple and rapid, the experimental cost is reduced, and the method is more conducive to monitoring the blood concentration of the clonazepam in a patient during clinical treatment.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring of psychotropic drugs, in particular to a method for detecting the concentration of clonazepam, an antiepileptic and anticonvulsant drug in blood. Background technique [0002] Epilepsy is a common neurological disease characterized by sudden and recurrent seizures. Currently, drug therapy is the first choice in clinical practice. Clonazepam is a broad-spectrum antiepileptic drug, used to control various types of epileptic seizures, and has a better effect on absence seizures, infantile spasms, myoclonic and akinetic seizures. It can also be used in the treatment of drug-induced attention deficit disorder (hyperactivity disorder) and various neuralgia. The active compound circulating in the blood is clonazepam after it passes through the gastrointestinal tract after oral administration. Clonazepam (Clonazepam, CZP), the molecular formula is C 15 h 10 ClN 3...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 赵金宝许丽贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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