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Preparation method of compound microbial agent for aquaculture

A composite bacterial agent and aquaculture technology, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of poor biological activity and adaptability, poor controllability of the fermentation process, and survival rate of beneficial bacteria and low content, to enhance nutritional diversity, inhibit algae reproduction, biological activity and strong adaptability

Inactive Publication Date: 2018-08-21
江西润垚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of aquaculture composite bacterial agent, which reasonably solves the problem that prior art aquaculture composite bacterial agent is prepared by conventional fermentation process, the fermentation process is poorly controllable, and the survival rate and content of beneficial bacteria are low. , the problem of poor biological activity and adaptability

Method used

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Embodiment

[0020] A method for preparing a composite bacterial agent for aquaculture, comprising nano soybean powder, agar, straw powder, Bacillus subtilis, Bacillus coagulans, lactic acid bacteria, nitrifying bacteria, amino acid enzyme bacteria, white granulated sugar, characterized in that,

[0021] The preparation method of described a kind of aquaculture compound bacterial agent comprises the following steps:

[0022] Step 1, preparation of inoculation base: Agar is made into a square with a length of 6 cm and a thickness of 3 cm. On the square, 5 rows of inoculation holes with a spacing of 1 cm are punched from one end edge 1 cm, and subtilis is inoculated successively in the inoculation holes. Bacillus, Bacillus coagulans, lactic acid bacteria, nitrifying bacteria, amino acid enzyme bacteria, cultivated at room temperature for 72 hours to prepare the inoculum,

[0023] Step 2, preparation of culture medium: mix the nano-soybean powder crushed to a particle size of less than 50 nan...

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PUM

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Abstract

The invention discloses a preparation method of a compound microbial agent for aquaculture. The preparation method is characterized by comprising steps as follows: S1, preparation of an inoculation medium; S2, preparation of a culture medium; S3, respective domestication culture; S4, mixed domestication culture; S5, preparation of the compound microbial agent for aquaculture. Adopted nano soybeanmeal has high nutritional value and is matched with straw powder to increase the content of cellulose, a matrix contains comprehensive nutritional ingredients, and growth of probiotics is facilitated.Through multi-round domestication breeding, the microbial agent has high survival rate and content of flora and high bioactivity and adaptability and has the characteristics of efficiency, stabilityand rapid propagation, harmful substances are decomposed by probiotics in a disease-resistant manner, healthy growth of aquatic products is enhanced, harmful substances such as ammonia, nitrogen and the like in water are degraded, the removal rate reaches 97% or above, breeding of algae can be inhibited, bacterial florae in the water can be rapidly recovered and balanced, the water is purified, the environment is improved, and defects in the prior art are overcome.

Description

technical field [0001] The invention relates to the technical field of biological bacterial agents, in particular to a method for preparing aquaculture composite bacterial agents. Background technique [0002] The prior art aquaculture compound bacterial agent is prepared by a conventional fermentation process, the fermentation process is poorly controllable, the survival rate and content of beneficial bacteria are low, and the biological activity and adaptability are poor. [0003] The present invention adopts soybean powder with broken cell wall to have high nutritional value, and the nano-fine crushing of broken cell wall can fully maintain the biological activity of trace elements in the raw material, enhance the nutritional diversity of the matrix, add straw powder to increase the cellulose content, and increase the nutritional value of the matrix. The ingredients are comprehensive and conducive to the growth of probiotics. Multiple rounds of domestication and reproduc...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C12N1/16C02F3/34C12R1/125C12R1/07C02F103/20
CPCC02F3/341C02F3/347C02F2103/20C12N1/16C12N1/20C12N1/36
Inventor 张友超朱红霞
Owner 江西润垚生物科技有限公司
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