Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition and culture medium containing the same

A culture medium and ph value technology, applied in the field of microbial cultivation, to achieve the effect of solving long-term storage and improving storage time

Active Publication Date: 2021-11-26
SHENZHEN BLOT BIOTECH +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This medium can greatly increase the storage time of Helicobacter pylori under different low temperature conditions, effectively solving the problem of long-term storage of Helicobacter pylori strains

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition and culture medium containing the same
  • Composition and culture medium containing the same
  • Composition and culture medium containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] formula:

[0034]

[0035] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.2, and sterilize under high pressure in a high-pressure steam sterilizer at 121°C for 20 minutes.

Embodiment 2

[0037] formula:

[0038]

[0039] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.3, and sterilize under high pressure in a high-pressure steam sterilizer at 121°C for 20 minutes.

Embodiment 3

[0041] formula:

[0042]

[0043] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.1, and sterilize under high pressure in a high-pressure steam sterilizer at 121°C for 20 minutes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of microorganism cultivation, in particular to a composition and a culture medium containing the composition. The invention provides a composition and a preservation medium containing the composition including bovine heart extract, bovine brain extract, cysteine, Na 2 HPO 4 , peptone and NaCl. Through the proportion of composition, buffer system and cell protection agent, the storage time of Helicobacter pylori can be greatly improved under different low temperature conditions, and the problem of long-term storage of Helicobacter pylori strains can be effectively solved, especially suitable for Helicobacter pylori specialty The strain library requires long-term strain preservation.

Description

technical field [0001] The invention relates to the field of microorganism cultivation, in particular to a composition and a culture medium containing the composition. Background technique [0002] Helicobacter pylori or Helicobacter pylori, English name Helicobacter pylori, referred to as Hp. Gram-negative, microaerophilic bacteria that live in various regions of the stomach and duodenum. It can cause mild chronic inflammation of the gastric mucosa and even lead to gastric and duodenal ulcers and gastric cancer. Helicobacter pylori is a spiral-shaped, micro-anaerobic bacteria that requires very harsh growth conditions. In 1983, it was successfully isolated from the gastric mucosal biopsy tissue of patients with chronic active gastritis for the first time. It is the only microbial species known to be able to survive in the human stomach. Epidemiological analysis shows that the infection rate is as high as 50% to 70% worldwide. The detection rate of HP in chronic active g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/04C12N1/20
Inventor 王洪涛林骏刘尧马伟民张永顶刘谦
Owner SHENZHEN BLOT BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com