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Molecular marking method related to soybean hundred-grain weight and marker combination acquired thereby

A molecular marker, 100-grain weight technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., to reduce the workload of high-generation breeding selection, reduce breeding scale, and shorten breeding years.

Inactive Publication Date: 2018-08-17
CROP RES INST SHANDONG ACAD OF AGRI SCI
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Problems solved by technology

However, since quantitative traits such as 100-seed weight are controlled by minor polygenes, these markers are specific among populations and cannot be used for molecular marker-assisted selection breeding in specific breeding populations.

Method used

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  • Molecular marking method related to soybean hundred-grain weight and marker combination acquired thereby
  • Molecular marking method related to soybean hundred-grain weight and marker combination acquired thereby
  • Molecular marking method related to soybean hundred-grain weight and marker combination acquired thereby

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Embodiment 1

[0021] Embodiment 1, a molecular marker method related to soybean hundred-grain weight, comprising the following steps:

[0022] 1) The 100-seed weight distribution of soybean RIL populations: Huapidou and Qihuang 26 high-generation RIL populations were planted for two consecutive years, and the 100-seed weights were counted. It was found that the 100-seed weights of the RIL populations were basically in a normal distribution. like Figure 1-2 shown.

[0023] 2) QTL mapping for 100-seed weight in soybean: The high-density genetic map constructed based on SLAF-seq technology was used to map the QTL of 100-seed weight in soybean RIL population, and a total of 10 soybean 100-seed weight were obtained by using complete interval mapping (ICIM). Related QTL loci ( image 3 ), located on chromosomes 5, 5, 9, 11, 11, 12, 13, 15, 20, and 20 (Table 1), explaining 93.81% of the phenotypic variation. like image 3 shown.

[0024] Table 1 QTL loci related to 100-kernel weight in soybe...

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Abstract

The invention relates to a molecular marking method related to soybean hundred-grain weight, comprising the steps of 1) acquiring distribution of soybean RIL (recombinant inbred line) populations, tobe specific, subjecting advanced RIL populations of glossy-skin soybean and Qi Huang 26 to continuous two-year planting, and counting their hundred-grain weight; 2) performing QTL (quantitative traitloci) positioning on soybean hundred-grain weight, to be specific, using a high-density genetic map constructed via SLAF-seq (specific-locus amplified fragment sequencing) technique to perform QTL positioning on the hundred-grain weight of the soybean RIL populations so as to obtain 10 QTL related to the hundred-grain weight. Low-generation marker selection of breeding materials by the marker combination helps gather heavy-grain excellent genes in the breeding materials at low generation, advanced breeding selection work is reduced, breeding scale is narrowed, and breeding time is shortened.

Description

technical field [0001] The invention relates to the technical field of molecular marker assisted breeding, in particular to a molecular marker method related to 100-seed weight of soybean and marker combination thereof. Background technique [0002] Soybean is an important grain, oil and feed crop in my country, and has an important strategic position in the development of the national economy. Soybean kernel weight is an important component of soybean yield, and it has a significant positive correlation with soybean yield in previous studies, and it is an important target for breeding high-yielding soybean varieties. However, soybean kernel weight is affected by different genetic backgrounds and fluctuates greatly between years, which is one of the main reasons for the slow progress in high-yielding soybean breeding. [0003] At present, there are 265 QTLs related to soybean grain weight included in the SoyBase database. However, since quantitative traits such as 100-seed...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张彦威李伟林延慧张礼凤王彩洁徐冉
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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