MTHFR (methylenetetrahydrofolate reductase) gene fragment as SNP (single-nucleotide polymorphism) molecular marker associated with sow foot-leg bone density

Abstract

The invention belongs to the technical field of animal molecular marking and particularly relates to MTHFR (methylenetetrahydrofolate reductase) gene fragment as an SNP (single-nucleotide polymorphism) molecular marker associated with sow foot-leg bone mineral density. The molecular marker is cloned from the MTHFR gene fragment having the register number of M1GA0008657; the gene fragment is subjected to typing screening via a gene chip technology so as to obtain the molecular marker associated with sow foot-leg bone mineral density; a nucleotide sequence of the molecular marker is shown as inSEQ ID NO: 1; base 51 of the nucleotide sequence has an A / C allele mutation; when nucleotide 51 in the SEQ ID NO: 1 is A, sow leg-foot bone has higher bone mineral density. Disclosed a method of screening molecular markers associated with sow pig-leg bone mineral density, as well as application of association analytical method. The novel SNP molecular marker is provided for marking-assisted selection of pig leg-foot compactness traits, particularly bone mineral density.

Description

technical field [0001] The invention relates to the technical field of preparation of pig molecular markers, in particular to the use of MTHFR gene fragments as SNP molecular markers related to bone density of sows' limbs and hooves. The molecular marker of the invention is cloned from the MTHFR gene, and can be used to predict the bone density traits of pig limbs and hooves. Background technique [0002] With the development of science and technology, the pig breeding industry has made significant breakthroughs in genetic breeding, nutritional regulation, and disease prevention and control. The lean meat rate, feed-to-meat ratio, and growth rate of commercial pigs have all been greatly improved. However, with intensive farming comes the increasingly prominent problem of limbs and feet. For intensive production, the range of activities of pigs is limited. In addition, the design of pens (slatted floors) has exacerbated the occurrence of pigs' limbs and feet. It has been rep...

AI Technical Summary

Problems solved by technology

It can be seen that bone density is an important indicator in the study of animal bone strength, but due to the limitations of various production conditions, people's research on livestock bones is very rare

[0004] Bone mineral density, that is, the content of bone mineral per unit volume, the unit is g / cm3, common bone density measurement methods include radioabsorption method, dual-energy X-ray measurement method, quantitative computed tomography technology and axial ultrasonic transmission technology, etc. (Cao Jian, Application evaluation of bone mineral density (BMD) measurement[J], Frontiers of Medicine, 2013(2)), among which dual-energy X-ray measurement and quantitative computed tomography technology are the most widely used, but dual-energy X-ray measurement technology is the most accurate Bone density is area, affected by the geometric volume of the object, and quantitative computed tomography can measure bone density in animals more accurately, but its equipment is expensive (Liu Jun, dual-energy X-ray absorptiometry and quantitative CT to measure bone density Comparative study [D]. Journal of Central South University, 2005), and the operation is very troublesome (for large livestock, it needs to be performed after anesthesia), which greatly limits its application in animal bone density research

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