Eel ferment, and preparation method and application thereof
An eel and enzyme technology, applied in the directions of bacteria, application, climate change adaptation, etc. used in food preparation, can solve problems such as unreasonable mix of beneficial bacteria types and quantities, insufficient fermentation technology, and insufficient fermentation of raw materials, etc. Protein utilization and conversion rate, beneficial to digestion and absorption, and the effect of improving the success rate of breeding
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[0044] The invention also discloses a preparation method of eel enzyme, comprising the following steps:
[0045] Step 1. Prepare the fermentation bag:
[0046] Step 1.1, weighing: Weigh the following components according to parts by mass: 70-80 parts of lactic acid bacteria, 5-15 parts of bacillus, 10-20 parts of yeast;
[0047] Step 1.2, starter preparation: take lactic acid bacteria, bacillus and yeast strains to carry out seed culture respectively, and prepare lactic acid bacteria seed culture liquid, bacillus seed culture liquid and yeast seed culture liquid;
[0048]Step 1.3, using graded fermentation: add the Bacillus seed culture solution and yeast seed culture solution into the fermenter for aerobic fermentation, control the temperature at 25-35°C for 10-24 hours, and stop the fermentation when the OD value is greater than or equal to 0.5 Adding lactic acid bacteria seed culture liquid to the fermenter for anaerobic fermentation, controlling the temperature at 32-38°C...
Embodiment 1
[0058] A preparation method of eel enzyme, comprising the following steps:
[0059] Step 1. Prepare the fermentation bag:
[0060] Step 1.1, weighing: Weigh the following components according to parts by mass: 75 parts of lactic acid bacteria, 10 parts of bacillus, and 15 parts of yeast;
[0061] Step 1.2, starter preparation: take lactic acid bacteria, bacillus and yeast strains to carry out seed culture respectively, and prepare lactic acid bacteria seed culture liquid, bacillus seed culture liquid and yeast seed culture liquid;
[0062] Step 1.3, using graded fermentation: add the bacillus seed culture solution and the yeast seed culture solution into the fermenter for aerobic fermentation, control the temperature at 30°C for 18 hours, stop the fermentation when the OD value is greater than or equal to 0.5; Add lactic acid bacteria seed culture liquid into the tank for anaerobic fermentation, control the temperature at 35°C for 18 hours, stop the fermentation when the OD i...
Embodiment 2
[0072] A preparation method of eel enzyme, comprising the following steps:
[0073] Step 1. Prepare the fermentation bag:
[0074] Step 1.1, weighing: Weigh the following components according to parts by mass: 70 parts of lactic acid bacteria, 15 parts of bacillus, and 15 parts of yeast;
[0075] Step 1.2, starter preparation: take lactic acid bacteria, bacillus and yeast strains to carry out seed culture respectively, and prepare lactic acid bacteria seed culture liquid, bacillus seed culture liquid and yeast seed culture liquid;
[0076] Step 1.3, using graded fermentation: add the bacillus seed culture solution and yeast seed culture solution into the fermenter for aerobic fermentation, control the temperature at 25°C for 24 hours, stop the fermentation when the OD value is greater than or equal to 0.5; Add lactic acid bacteria seed culture liquid into the tank for anaerobic fermentation, control the temperature at 32°C for 24 hours, stop the fermentation when the OD is gr...
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