Light-sheet microscope

Abstract

The embodiments of the invention disclose a light-sheet microscope. The light-sheet microscope of the invention comprises a laser light source, a light wave phase adjuster for adjusting the phase of alaser beam, a light sheet generating device used for receiving the laser beam emitted by the light wave phase adjuster and shaping the laser beam into a light sheet, an excitation objective lens usedfor coupling the light sheet to a sample to excite the sample to generate fluorescence, an imaging objective lens used for collecting and concentrating the fluorescence generated by the excited sample, a photoelectric detection device used for imaging the fluorescence, a first beam splitter disposed between the imaging objective lens and the photoelectric detection device, a second beam splitterfor changing the light path of fluorescence emitted by a two-photon fluorescent spot, and a wavefront detector for detecting the wavefront distortion of the fluorescence emitted by the two-photon fluorescent spot. With the light-sheet microscope provided by the embodiments of the invention adopted, the wavefront distortion of the laser light sheet can be corrected, and the deep imaging of a turbidsample can be realized.

Description

technical field [0001] Embodiments of the present invention relate to the technical field of microscopes, and in particular to a light sheet microscope. Background technique [0002] The imaging method of the light sheet microscope is to select the illumination focal plane for imaging. This method can avoid exciting the sample outside the focal plane, so that the image is not disturbed by the out-of-focus signal, thereby realizing optical section tomography. Since the excited plane of the sample is the focal plane during imaging and will not affect other parts of the sample, light sheet microscopy can minimize photobleaching and photodamage, which is conducive to long-term imaging of living cells. [0003] Traditional light sheet microscopy has the limitation of insufficient penetration depth for turbid samples. When the excitation beam is focused on a biological sample with insufficient transparency, the beam will produce wavefront distortion, which will cause the light sh...

AI Technical Summary

Problems solved by technology

[0003] Traditional light sheet microscopes have the limitation of insufficient penetration depth for turbid samples

When the excitation beam is focused on a biological sample with insufficient transparency, the beam will produce wavefront distortion, which will cause the light sheet to become thicker, reduce the resolution and contrast of the image, and thus reduce the penetration depth of illumination

The existing practice is that when imaging the depth of a turbid sample, it is necessary to pre-transparify the sample, which will not only cause certain damage to the sample, but also be inconvenient

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