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A Breeding Method for Inducing Haploid by Using Yellow-green Seedling Marker-Assisted Selection in Maize Hybridization

A haploid induction system and marking technology, which is applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problems of wasting manpower, material and financial resources, and difficulties in haploid identification, so as to reduce manpower and save manpower , the effect of strong reliability

Active Publication Date: 2021-11-05
JILIN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the difficulty in identifying haploids in the screening markers used in maize hybridization-induced haploid breeding, waste of manpower, material resources, and financial resources, and may cause early natural doubled EDH lines to be eliminated as pseudo-haploid strains, The purpose of the present invention is to provide a breeding method using yellow-green seedling marker-assisted selection of maize hybridization to induce haploid

Method used

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  • A Breeding Method for Inducing Haploid by Using Yellow-green Seedling Marker-Assisted Selection in Maize Hybridization
  • A Breeding Method for Inducing Haploid by Using Yellow-green Seedling Marker-Assisted Selection in Maize Hybridization
  • A Breeding Method for Inducing Haploid by Using Yellow-green Seedling Marker-Assisted Selection in Maize Hybridization

Examples

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Effect test

Embodiment 1

[0042] Example 1 The breeding process of the haploid induced line Jihuangyu No. 7 with yellow-green seedling markers and its sister lines in the present invention.

[0043] (1) The dominant homozygous yellow-green seedling gene material 523DYg3-N1582 was introduced from the United States in 2011 (523DYg3-N1582 is the code name of this material in the American Maize Genetic Resources Collection Center, and the preservation number of this material in the US Department of Agriculture is: MGS 9012023, both available and available free of charge). And other inductive materials from the United States, the code names are: H08, S05:12, S05:26, S05:34, S05:40, S05:54 and S05:60.

[0044] (2) In the summer of 2012, 523DYg3-N1582 (female parent), haploid induction line Jigaoyuxi No. 3 (cultivated by Jilin Academy of Agricultural Sciences) and Jigaoyuline 31 were planted in the Jilin Academy of Agricultural Sciences No. (Cultivated by Jilin Academy of Agricultural Sciences), H08, S05:12,...

Embodiment 2

[0055] Example 2 Experiment of breeding maize inbred lines by hybridization induction with haploid induction lines with yellow-green seedling markers such as Jihuangyu No. 7

[0056] Proceed as follows:

[0057] (1) In 2014, three single-cross F 1 Generations; numbered 1, 2, and 3, respectively.

[0058] (2), in the summer of 2015, in Gongzhuling, Jilin Academy of Agricultural Sciences, the haploid induction lines (see Table 2) with the yellow-green seedling markers such as Jihuangyu No. 7 cultivated in Example 1 are male parents, respectively The F 1 Pollination was carried out in a "one-to-one" manner, and 28 spikes were harvested on the female plant; according to the traits of the grain markers (the grains whose top aleurone layer and germ tip were both purple were eliminated, they were diploid grains obtained by normal hybridization; The aleurone layer at the top is purple, and the tip of the germ is colorless, which is the induced parthenocarpy "quasi-haploid" grain) f...

Embodiment 3

[0069] Example 3: Haploid-inducing lines such as Jihuangyu No. 7 and other haploid-inducing lines were used to induce haploid breeding of hybrids to test DH lines of maize

[0070] Proceed as follows:

[0071] (1), in the winter of 2015, in Nanfan District, Jilin Academy of Agricultural Sciences, Ledong, Hainan, two commercial hybrids of Dika 516 and Syngenta 203 (purchased in the seed market in 2015) were used as the basic population, and the examples were taken The haploid induction line Jihuangyu No. 7 with yellow-green seedling markers bred in 1 or its sister line was used as the male parent, and Dika 516 and Syngenta 203 were used for "one-to-one" single-ear pollination, and the harvest On the ear of the female parent plant, the "quasi-haploid" grain is screened according to the traits of the grain markers that the top aleurone layer is purple and the germ tip is colorless, and the quasi-haploid induction rate of the grain is calculated. The calculation formula is:

[00...

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Abstract

The invention discloses a breeding method for using yellow-green seedling markers to assist selection of maize hybridization induced haploids, and belongs to the field of breeding methods. The present invention utilizes the induced lines with yellow-green seedling markers such as Jihuangyu No. 7 to pollinate the basic population and harvest fruit ears; at the seedling stage, the induced haploids are identified and screened according to the color of the leaves, and the hybrids whose leaves are yellow-green are eliminated. Synthesis of diploid plants, haploid plants with green leaf color or early doubled haploid plants, selfing at flowering stage to obtain new inbred lines. The yellow-green seedling marker used in the method of the present invention is controlled by a single dominant gene. Compared with grain markers and adult plant markers, the genetics are simple, and the distinguishing features are obvious. The identification greatly saves manpower and material resources, is suitable for large-scale induced haploid breeding, and has high breeding efficiency, especially for accurate identification of early doubled haploids, and realizes one-step line formation.

Description

Technical field: [0001] The invention belongs to the field of maize haploid breeding methods, and in particular relates to a breeding method for using yellow-green seedling markers to assist selection of maize induced haploids. Background technique [0002] It usually takes 10 to 15 years to breed a corn variety by using traditional hybrid breeding methods. Not only is the cycle long, the scale is large, time-consuming and labor-intensive, but it may also take too long, and the breeding goals set at the initial stage of breeding may no longer exist when the variety is bred. Meet the requirements of the market. Therefore, shortening the breeding time and accelerating the breeding speed have always been the goals that breeders strive for. In the 1970s, haploid breeding methods such as anther culture or pollen culture were once regarded as promising methods due to their short breeding cycle. Promote apps. [0003] In 1959, Coe E H of the University of Minnesota in the United...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 才卓徐国良代玉仙任军李淑华于明彦刘小丹郭琦李月英王丽娜张铭堂
Owner JILIN ACAD OF AGRI SCI
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