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A method for identifying the properties of phloem juice

A technology of phloem and sap, applied in the field of plant sap identification, can solve the problems of inaccuracy and wrong judgment, and achieve the effect of simple operation, low cost and simple gene

Active Publication Date: 2021-12-14
广东省农业科学院农业质量标准与监测技术研究所
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Problems solved by technology

When judging by this method, when the phloem juice is slightly polluted, the non-reducing sugar content is still at a low level, which leads to misjudgment, making it inaccurate

Method used

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  • A method for identifying the properties of phloem juice
  • A method for identifying the properties of phloem juice
  • A method for identifying the properties of phloem juice

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Experimental program
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Embodiment 7

[0033] According to the experimental condition of embodiment 2, adopt above-mentioned test mode to carry out experiment to different plants, (wherein select suitable nutrient solution according to the plant that is used for experiment), obtain R sap / R plant value, so as to judge whether the phloem juice is polluted, and then use genetic assay to test the results. The specific results are shown in the table below:

[0034]

[0035]

[0036] As can be seen from the results in the above table, using the difference in the content ratio of rubidium / strontium in the phloem and other plant tissues, that is, R sap / R plant The value can accurately judge whether the phloem juice is polluted, which is beneficial to further research on the phloem juice.

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Abstract

The invention discloses a method for identifying the properties of phloem juice, which is characterized in that: adding strontium salt and rubidium salt in the process of plant cultivation; then extracting the plant phloem juice from the plant tissue; respectively measuring the phloem juice and the plant The content of strontium element and rubidium element in the tissue; the content ratio of rubidium element / strontium element in the phloem extract (R sap ), and the content ratio of rubidium / strontium in the plant tissue (R plant ) for comparison, when R sap / R plant ≥6, it is judged that the phloem juice is not polluted. Compared with the existing identification methods, the method of the invention has simple operation, high accuracy and low consumption of phloem extract.

Description

technical field [0001] The invention relates to the technical field of plant juice identification, in particular to a method for identifying the properties of phloem juice. Background technique [0002] Phloem transport is an important physiological process in plants, and it is of great significance to the transport of carbohydrates and mineral elements in plants. Therefore, the phloem juice has important research value for the physiology, biochemistry, nutrition and pathology of plants. The extraction methods of phloem juice include injury flow method, EDTA stimulation extraction method and so on. The phloem extract obtained by these methods is generally small in amount and may be polluted by xylem juice or cell fluid. Therefore, the nature of the extracted phloem juice must be identified to determine whether it is polluted. The main identification methods adopted at this stage include: [0003] Gene determination method: determine the degree of purity by determining whe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N27/626
CPCG01N21/31G01N27/62
Inventor 邓腾灏博杜瑞英文典黄永东吴志超李富荣徐守俊石含之李蕾
Owner 广东省农业科学院农业质量标准与监测技术研究所
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