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A Breeding Method Using Yellow-green Seedling Markers to Breed Early Doubled Haploids in Maize

A technology for doubling haploid, yellow-green seedlings, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the waste of manpower, material resources, financial resources and time, poor haploid grain identification accuracy, identification Time delay and other problems, to achieve the effect of avoiding light shading, saving manpower, and reducing ineffective labor

Active Publication Date: 2021-08-24
JILIN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] The existence of the Navajo purple spot marker used in the current maize hybridization induced haploid breeding is greatly affected by the maternal parent, especially in the presence of suppressed genes, the accuracy of identifying haploid grains is poor and difficult, and it is greatly affected by it The identification accuracy rate of pseudo-haploid grains of the basic material is less than 50%, and the identification by experience at the seedling stage may cause the risk that the excellent single plant (EDH line) with strong growth potential due to early doubling will be eliminated as pseudo-haploid, and the risk of adult The identification of the plant stage by purple leaves and purple stalks is late, which causes a lot of manpower, material resources, financial resources and time waste. The purpose of the present invention is to provide the yellow-green seedling traits as markers for the use of maize hybridization induced haploid breeding

Method used

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  • A Breeding Method Using Yellow-green Seedling Markers to Breed Early Doubled Haploids in Maize
  • A Breeding Method Using Yellow-green Seedling Markers to Breed Early Doubled Haploids in Maize
  • A Breeding Method Using Yellow-green Seedling Markers to Breed Early Doubled Haploids in Maize

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1 Genetic analysis and positioning of the yellow-green seedling gene Yg3 of the present invention

[0041] (1) In the summer of 2012, the material code-named 523DYg3-N1528 was planted in Gongzhuling, Jilin (or called, quoted from the American Corn Genetic Resources Conservation Center in 2011, the same below); 523DYg3-N1528 was pollinated by the pollen of Gaoyu Line 31 (all bred by Jilin Academy of Agricultural Sciences) and obtained F 1 Generation combinations (523DYg3-N1528╳Jigaoyu Line 3 is coded as Combination 1, 523DYg3-N1528╳Jigaoyu Line 31 is numbered as Combination 2) each with 2 spikes.

[0042] (2) In the winter of 2012, F 1 Substitute 1 line each, F 1 The first generation seedlings began to show very obvious yellow-green leaves at the 3-5 leaf age (light green seedlings, light green plants, yellow-green leaves, mainly yellow, supplemented by green), and Jigaoyu 3 and Ji The leaf color difference of Gaoyin 31 was extremely large, and the green co...

Embodiment 2

[0048] Embodiment 2 Utilizes the backcrossing method to cultivate the haploid induction line test with the yellow-green seedling marker

[0049] Proceed as follows:

[0050] (1) In the summer of 2012, 523DYg3-N1528 (non-recurrent parent) with yellow-green seedling markers and the haploid induction line Jigaoyue Line 3 (recurrent parent) were planted at the same time in Gongzhuling City, Jilin Province. 523DYg3-N1528 is yellow-green at the seedling stage and grows normally. After heading, the difference between yellow-green and normal green plants gradually becomes smaller; at the flowering stage, 523DYg3-N1528 is pollinated with Jigaoyuxi 3, and the hybrid F 1 generation seeds.

[0051] (2) In the winter of 2012, F was planted in Hainan 1 generation, at the same time, plant Jigaoyu Line 3; use Jigaoyu Line 3 as the male parent in the flowering stage, and give F 1 Pollination of strong and healthy plants; when harvesting, mix and thresh and select grains with obvious Navajo ...

Embodiment 3

[0061] Example 3: Breeding early doubled haploid experiment of maize using Jihuangyouxi No. 7 and its sister lines

[0062] Proceed as follows:

[0063] (1) In 2016, in Gongzhuling, Jilin Academy of Agricultural Sciences Haploid Seed Nursery, two commercial products were Jingke Nuo 928 (gifted by Beijing Academy of Agriculture and Forestry Sciences) and Yikou Sanxiang (gifted by Shandong Denghai Seed Industry). Fresh corn hybrids were used as the basic population, and the haploid induction line Jihuangyuxian No. 7 and its sister lines (see Table 3 and Table 4) cultivated in Example 2 with yellow-green seedling markers were used as male parents, respectively. Jingke Nuo 928 and Yikou Sanxiang were pollinated "one-to-one" for a single ear, and the upper ear of the female plant was harvested, and the "quasi-haploid" was screened according to the Navajo marker traits of the aleurone layer at the top and colorless germ tip. grain.

[0064] (2) In the winter of 2016, at the base o...

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Abstract

The invention discloses a method for breeding early doubled haploids of maize by using yellow-green seedling markers, and belongs to the field of breeding methods. The invention also discloses a method for identifying the induced haploid by using the yellow-green shoot marker and a method for cultivating the haploid induction line with the yellow-green shoot marker. The present invention uses the yellow-green seedling traits controlled by the single dominant gene Yg3 gene to realize accurate identification and screening of induced haploids at the seedling stage. Simple, with obvious distinguishing features, accurate and reliable identification of haploid, early doubled haploid, and pseudo-haploid can be made at the seedling stage, especially for the identification of doubled haploid, avoiding it as a pseudo-haploid Elimination can achieve one-step line formation; the use of yellow-green seedling markers has high identification efficiency, which greatly saves manpower and material resources, can be used to expand the size of the induced population, and can realize mechanized identification of seedlings, greatly improving breeding efficiency.

Description

Technical field: [0001] The invention belongs to the field of selective markers for maize hybridization-induced haploids, and specifically relates to the use of yellow-green seedling traits as markers in maize hybridization-induced haploid breeding; and also relates to the use of yellow-green seedling trait markers to identify maize hybridization-induced haploids The method; also relates to a breeding method for breeding early doubled haploids of maize by using yellow-green seedling markers. Background technique [0002] It usually takes 10 to 15 years to breed a corn variety by using traditional hybrid breeding methods, which is not only long-term, large-scale, time-consuming and labor-intensive, but also may be due to the long duration. Does not meet the requirements of the market. Therefore, shortening the breeding time and accelerating the breeding speed have always been the goals that breeders strive for. In the 1970s, haploid breeding methods such as anther culture o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 才卓徐国良任军代玉仙于明彦李淑华郭琦刘小丹李月英杨琇涵王丽娜张铭堂
Owner JILIN ACAD OF AGRI SCI
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