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Molecular markers and their application of the main effect qtl loci of peanut kernel yield

A molecular marker, peanut technology, applied in the field of molecular genetics and breeding, can solve the problems of difficult-to-flower breeding, low effect value, unknown stability, etc., and achieve the effect of improving breeding efficiency

Active Publication Date: 2019-05-24
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the QTL mapping of peanut kernel rate, the number of detected QTLs is small, the effect value is low, the stability of expression in different environments is unknown, and it is difficult to apply in peanut breeding

Method used

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  • Molecular markers and their application of the main effect qtl loci of peanut kernel yield
  • Molecular markers and their application of the main effect qtl loci of peanut kernel yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Test materials: The cultivated peanut variety Yuanza 9102 with a high kernel yield was used as the female parent, and the variety Xuzhou 68-4 with a low kernel yield was used as the male parent, and the recombinant peanut variety containing 195 lines was obtained by single-seed transmission method. Cross-line (RIL) population F 5 (2013), F 6 (2014), F 7 (2015) and F 8 (2016) s.

[0036] Phenotype identification: From 2013 to 2016, Yuanza 9102, Xuzhou 68-4 and F 5 (2013), F 6 (2014), F 7 (2015), F 8(2016) generated 195 strains from the RIL population. A completely randomized block experimental design was adopted, with 3 repetitions. Each time, each material was planted in 1 row with 12 plants, the row spacing was 30 cm, and the plant spacing was 20 cm. Adopt standard field management practices. The pods of 8 typical plants were harvested after maturity, and the kernel rate (seed weight÷pod weight×100%) was counted.

[0037] Construction of genetic linkage map:...

Embodiment 2

[0044] The 195 F markers of Yuanza 9102 and Xuzhou 68-4 were detected using four molecular markers linked to the main QTL locus cqSPA09 for peanut kernel yield. 7 (2015) strains were tested, and the individual plants that were all heterozygous for the four markers were detected. In these individual plants, the main QTL locus cqSPA09 for kernel rate was in a heterozygous state, while other genetic backgrounds were relatively close. These heterozygous individual plants are called residual heterozygous, and their selfed offspring constitute the residual heterozygous line (RHL) population. In the RHL population, use one or more of the four molecular markers linked to the main effect QTL site cqSPA09 of the peanut kernel rate for detection. If there is an amplified fragment identical to that of Yuanza 9102, it indicates that the plant is The kernel yield is high, and the absence of it indicates that the kernel yield is low. At the same time, it was verified by the actual measured...

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Abstract

The invention belongs to the field of molecular genetic breeding and particularly relates to molecular markers for peanut kernel yield major QTL (quantitative trait locus) and application thereof. Themolecular markers include Ad09A7577, AGGS1606, AHGA98567 and Ad91I24; one or more of primer pairs of the molecular markers Ad09A7577, AGGS1606, AHGA98567 and Ad91I24 are used to perform PCR (polymerase chain reaction) amplification on DNA of peanut material under identification, the amplified product is subjected to electrophoretic detection; if a DNA fragment of corresponding size is obtained byamplifying, it is shown that kernel yield major QTL cqSPA09 is present, and pods have high kernel yield.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and in particular relates to a molecular marker of the main effect QTL site cqSPA09 of peanut kernel yield rate and an application thereof. Background technique [0002] Peanut (Arachis hypogea L.) is one of the four major oil crops in the world and plays an important role in human dietary nutrition. Peanut production plays an important role in ensuring the limited supply of edible vegetable oil and protein in my country. In peanut production, peanuts are harvested and stored in pods. Peanut pods consist of two parts: the shell and the seed. Peanut seeds are rich in edible oils, proteins, amino acids and vitamins, and can be processed into edible oils, nuts, peanut butter and other foods. Peanut kernel yield refers to the percentage of seed weight in pod weight; the higher the kernel yield, the more parts that can be used for food processing. Therefore, kernel yield is one of the imp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 罗怀勇任小平黄莉陈玉宁周小静陈伟刚姜慧芳
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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