High-density molecular-marker-assisted pyramiding breeding method based on core genealogy varieties
A molecular marker-assisted, polymerization breeding technology, applied in the fields of botany equipment and methods, biochemical equipment and methods, plant genetic improvement, etc. Effects of breeding, reducing blindness and randomness, reducing breeding costs
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Embodiment 1
[0048] 1. Determine the genome segment information related to the traits of the core pedigree varieties.
[0049] Using the research method in section (4) of the background technology, more than 1,000 genome segments related to traits of the South China rice core pedigree variety "Huanghuazhan" were determined. In the subsequent analysis and processing, these genome segments are considered as the smallest recombination unit and are closely related to breeding.
[0050] The literature [Pedigree-based analysis of derivation of genome segments of anelite rice reveals key regions during its breeding. Plant Biotechnology Journal, 2016, 14(2): 638-648] introduced in detail the method of population resequencing combined with recombination breakpoint map (bin map) analysis, QTL mapping, and cloned gene information to determine the research methods of the associated segments of "Huang Huazhan" traits. Examples of related results are as follows figure 1 shown.
[0051] 2. Obtain the ...
Embodiment 2
[0098] In this example, the new-generation core pedigree variety Huangguangyouzhan derived from Huanghuazhan was used as the female parent, the introduced variety 1 was Wuguangzhan, and the introduced variety 2 was Fengyue Huazhan. Huangguangyouzhan is highly resistant to rice blast, the rice quality is not high-quality, and moderately sensitive to bacterial blight; Wuguangzhan is resistant to rice blast, with national standard high-quality grade 3 and provincial standard high-quality grade 2, and moderate resistance to bacterial blight; Fengyuehua Accounting: medium resistance to rice blast, rice quality national standard provincial standard high-quality grade 2, medium resistance to bacterial blight. The purpose of breeding is to maintain the high yield and rice blast resistance of Huangguangyouzhan, introduce the high-quality rice traits and bacterial blight resistance of Wuguangzhan and Fengyue Huazhan, and select high-yield, high-quality, and disease-resistant conventional...
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