Artificial culture method of perigord truffle strains
A technology of artificial cultivation, truffle bacteria, applied in the field of agriculture
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[0014] A kind of artificial cultivation method of black truffle strain mainly carries out according to the following steps: 1. Strain preparation: peel the mature black truffle thalline, extract the thalline entity and grind it into powder to be sporophyte or sporophyte aggregate 2. Culture medium preparation: 40-50 parts of dry corn stalk residue, 20-30 parts of sawdust powder, 30-40 parts of grass clippings, 20-30 parts of dry clay, 15-20 parts of fine sand, cow dung 8 to 10 parts, and mixed evenly to become the culture material; 3. Preparation of the cultivation greenhouse: the cultivation greenhouse, the light transmittance should be shaded with the increase of sunlight, and the shaded light intensity should be maintained. The depth of the base material is 0.5-0.6 meters; 4. Culture medium sterilization: heat the culture medium to 70°C-80°C for one minute and then naturally lower the temperature to 20°C-30°C; 5. Filling and planting the culture medium: Fill the high-temper...
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