Method for separating high-purity saponin monomers from tea seeds

Abstract

The invention specifically relates to a method for separating high-purity saponin monomers from tea seeds, belonging to the field of separation and purification of natural compounds. In particular, the method involves separation and purification of six high-purity pentacyclic triterpene oleanane saponins in tea seeds. Compared with the prior art, the method provided by the invention has the following beneficial effects: (a) the purity of the compounds are high, as high as 91 to 99%; (b) separation process is simple, and six main monomeric saponin substances in tea seeds can be obtained at thesame time; and (c) the method has good repeatability and is well targeted.

Description

technical field [0001] The present invention relates to the field of separation, purification and preparation of natural compounds, in particular to a method for separating high-purity tea seed saponin monomer compounds, and relates to the preparation of six high-purity pentacyclic triterpene oleanane-type saponins in tea seeds Isolation and Purification. Background technique: [0002] Tea (Camellia sinensis (L.) O.Kuntze) seeds are another important tea tree resource besides tea leaves. They are rich in triterpene saponins, which can regulate the stomach, antibacterial and anti-inflammatory, anti-tumor, protect nerves, promote plant absorption, Bioactivity and functions such as foaming and decontamination have great application prospects in the fields of food, medicine, agriculture, and chemical industry. The structure of saponin in tea seeds is similar in polarity, so it is very difficult to separate and prepare pure monomers. The lack of pure monomers seriously hinders ...

AI Technical Summary

Problems solved by technology

Its main disadvantages are large sample consumption, cumbersome operation in the separation process, especially the chromatographic part, poor repeatability, large blindness, and low separation efficiency