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A kind of plant growth regulator and culture method thereof for detached panicle of rice

A technology of plant growth regulators and cultivation methods, which is applied in the fields of plant growth regulators, plant growth regulators, botany equipment and methods, and can solve problems such as high cost, cumbersome operation, and bacterial infection

Active Publication Date: 2020-07-07
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the cost is high, the operation is cumbersome, and the nutrient solution needs to be replaced regularly and the pH value should be adjusted. Also, because the nutrient solution contains sugar and amino acids, it is very easy to cause bacterial infection and affect the success rate of isolated panicle culture.

Method used

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  • A kind of plant growth regulator and culture method thereof for detached panicle of rice
  • A kind of plant growth regulator and culture method thereof for detached panicle of rice

Examples

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Effect test

Embodiment 1

[0025] The present invention aims at the practical problems of low seed setting rate and low thousand-grain weight existing in isolated rice panicle culture, and adopts the method of applying hormone and glucose to isolated panicles and leaves to increase the absorption of hormones and nutrients by leaves and chaff, and improve the in vitro The grouting environment of the ear grains also increases the pollination life of the pistil stigma, prolongs the time for cross breeding operations, and achieves the purpose of increasing the seed setting rate and thousand-grain weight. Specifically, it is completed through the following steps:

[0026] 1) Sampling of isolated tassels. In the field, select healthy ears that have not yet headed (the ear bracts are full, and the development period is in the meiosis stage of rice pollen mother cells to the mature stage of pollen grains, which is about to head), and cut the whole stem from the base (root) of the plant, and quickly remove Put t...

Embodiment 2

[0031] In August 2017, at the China Rice Research Institute, three different nutrient media were used, and the isolated ears of rice Nipponbare were used as materials for in vitro culture. The nutrient solution in the nutrient solution culture method was prepared according to the rice nutrient solution formula of the International Rice Research Institute. , the detached ears headed and flowered in the incubator and completed self-pollination. The parameters such as seed setting rate and thousand-grain weight at harvest are shown in Table 1.

[0032] Table 1 Thousand-grain weight and seed-setting rate of in vitro panicle culture using different nutrient media

[0033]

[0034]

[0035] The data of table 1 shows, adopts the technology of the present invention to carry out the rice ear of detached panicle culture, and its seed setting rate, thousand grain weight and output are all significantly higher than nutrient solution culture method, also higher than tap water culture ...

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Abstract

The invention provides a plant growth regulator and a cultivation method for paddy rice in-vitro panicles. The plant growth regulator comprises gibberellin with the concentration of 5-10 mg / L, glucosewith the mass fraction of 0.1%-1.0% and tween-20 with the mass fraction of 0.01%-0.1%. The cultivation method includes sampling in-vitro panicles; carrying out artificial cutting on the in-vitro panicles; spraying or smearing the plant growth regulator on leaf surfaces; cultivating the in-vitro panicles in growth chambers. The plant growth regulator and the cultivation method have the advantagesthat the cultivation method includes processes for smearing hormone and the glucose on the in-vitro panicles and leaves, accordingly, hormone and nutrient absorption of the leaves and glumes can be improved, and grain filling environments for grains of the in-vitro panicles can be improved; the pollination lives of stigmas of pistils can be prolonged, accordingly, the hybridization breeding operation time can be prolonged, and the purpose of increasing the setting rates and the thousand-grain weights can be achieved.

Description

technical field [0001] The invention belongs to the technical field of rice cultivation, and in particular relates to a plant growth regulator for isolated rice panicles and a cultivation method thereof. Background technique [0002] Rice is one of the main grain crops in my country. The rice planting area accounts for about 30% of the total area of ​​grain crops in my country, but the total output accounts for 40% of the total grain output. China is a big country in rice production and consumption, and more than 50% of the population takes rice as a staple food. It is predicted that my country's population will reach 1.6 billion by 2030. Even if the area of ​​cultivated land does not decrease, the comprehensive production capacity of food crops will still need to increase by 55% to basically meet the needs of food. Rice will account for more than 40% of the increased food production. Under the national conditions of population increase and land decrease, cultivating super...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/22A01G2/10A01G7/06A01C1/00A01N43/16A01N43/12A01P21/00
CPCA01C1/00A01G7/06A01N43/12A01N43/16
Inventor 陶龙兴符冠富陈婷婷奉保华张彩霞符卫蒙李光彦
Owner CHINA NAT RICE RES INST
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