Molecular marker and application closely related to grain weight and silique length of oilseed rape
A technology of molecular markers and grain weight, which is applied in the field of rapeseed breeding and molecular biology, can solve the problems of low selection efficiency, long breeding cycle, and susceptibility to environmental influences, so as to reduce the workload, shorten the breeding period of rapeseed, and improve the quality of rapeseed breeding. efficiency effect
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Embodiment 1
[0030] A molecular marker BnaA.ARF18a-186 and BnaA.ARF18a-262 closely related to rape seed weight and silique length were obtained by the following method:
[0031] In this example, 157 rapeseed varieties with different genetic sources and large variations in grain weight and silique length were used as an example to construct a natural population, and describe in detail the method for obtaining molecular markers closely related to rapeseed grain weight and silique length. details as follows:
[0032] (1) Group construction:
[0033] A natural population of 157 rapeseed cultivars with different genetic origins and large variations in grain weight and silique length (1.75-5.71g / 4.02-10.62cm) was used as research materials (collected worldwide).
[0034] (2) Determination of grain weight and silique length:
[0035] (1) The plant material is planted in the field, with 3 lines per line, 8-10 plants in each line, random block design; the Yangtze River Basin is generally sown in ...
Embodiment 2
[0061] The application of a molecular marker closely related to rapeseed grain weight and silique length in high-yield breeding of rapeseed comprises the following steps:
[0062] Using the European winter variety Express (low-grain weight silique) and the Chinese semi-winter variety House7 (high-grain weight long silique) as parents, a DH segregation population was constructed as research materials, using BnaA.ARF18a-186 and BnaA.ARF18a -262 Two kinds of dCAPS markers further expand the detection range, the detection method is as follows:
[0063] (1) Extracting DH population and parental DNA, the method is the same as in Example 2.
[0064] (2) First detect the haplotype type of the parents of the DH population, and use the following primers to carry out the PCR process:
[0065] The forward primer sequence of BnaA.ARF18a-186 is 5'-ATGGCGAATGTAGATGGAGAT-3', and the reverse primer sequence is 5'-CTCTGTGTACAGCTGATCTTGATAT-3';
[0066] The forward primer sequence of BnaA.ARF1...
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