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Method for preparing small molecule donkey-hide gelatin peptide by using donkey-hide gelatin pieces

A gelatin peptide and small molecule technology, applied in the field of preparing small molecule gelatin peptides, can solve the problems of acid pollution, complicated steps, long operation process, etc., and achieve the effects of reducing the enzymatic hydrolysis time, improving the enzymatic hydrolysis degree and improving the yield.

Active Publication Date: 2018-07-20
UNIV OF JINAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ye Chuanfa et al. (Ye Chuanfa, Zhang Xiaodong, Hu Chengsi, et al. A method for extracting donkey-hide gelatin peptide: China, 102021215A[P]. , trypsin, flavor protease step by step enzymatic hydrolysis, decolorization by activated carbon, nanofiltration desalination concentration, spray drying preparation method, the operation process of this method is too long, the steps are cumbersome, the cost of trypsin is high, and it is not suitable for industrial production
Zhong Hongguang et al. (Zhong Hongguang, Yi Minzhi, Lu Jianzhong, et al. A preparation method of donkey-hide gelatin oligopeptide: China, 102321716A[P]. Protease, neutral protease, and flavor protease are enzymatically hydrolyzed step by step, and the donkey-hide gelatin peptide is obtained through membrane concentration. The raw material treatment process of this method is too cumbersome, and the step-by-step enzymatic hydrolysis takes a long time
Zhang Fan et al. (Zhang Fan, Huang Yanhong, Wang Yingying, et al. A method for preparing donkey-hide gelatin small-molecule low-peptide: China, 106831938A[P]. Pollution
[0006] The above method is cumbersome to process donkey-hide gelatin raw materials, multi-enzyme step-by-step enzymatic hydrolysis or high-pressure hydrolysis with acid, the process is cumbersome, the cost is high, and it is not suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] ① Compound enzymatic hydrolysis method to enzymatically hydrolyze donkey-hide gelatin

[0032] Add dried donkey-hide gelatin lumps into the buffer solution to prepare a concentration of 110g (dry) / L, and adjust the initial pH to 7.5; For protease, add 13000U per gram of dried donkey-hide gelatin, and enzymatically hydrolyze at 50°C for 110 minutes to obtain a hydrolyzate;

[0033] The dried donkey-hide gelatin lump is an intermediate product of the concentration process in the production process of donkey-hide gelatin, with a water content of 13.1% and a protein content of donkey-hide gelatin of 86.8%;

[0034] Described buffer solution is the disodium hydrogen phosphate-sodium hydroxide solution of pH7.5;

[0035] The compound enzyme method is to carry out synchronous enzymolysis after mixing the added alkaline protease and neutral protease with an enzyme activity unit of 1:4;

[0036] Described alkaline protease, neutral protease are food grade;

[0037] The constant...

Embodiment 2

[0045] ① Compound enzymolysis method to enzymatically hydrolyze donkey-hide gelatin

[0046] Add dried donkey-hide gelatin lumps into the buffer solution to prepare a concentration of 110g (dry) / L, and adjust the initial pH to 7.0; For protease, add 13000U per gram of dried donkey-hide gelatin, and enzymatically hydrolyze at 55°C for 110 minutes to obtain a hydrolyzate;

[0047] The dried donkey-hide gelatin lump is an intermediate product of the concentration process in the production process of donkey-hide gelatin, with a water content of 13.1% and a protein content of donkey-hide gelatin of 86.8%;

[0048] Described buffer solution is the disodium hydrogen phosphate-sodium hydroxide solution of pH7.5;

[0049] The compound enzyme method is to carry out synchronous enzymolysis after mixing the added alkaline protease and neutral protease with an enzyme activity unit of 1:4;

[0050] Described alkaline protease, neutral protease are food grade;

[0051] The constant temper...

Embodiment 3

[0059] ① Compound enzymolysis method to enzymatically hydrolyze donkey-hide gelatin

[0060] Add dried donkey-hide gelatin lumps into the buffer solution to prepare a concentration of 110g (dry) / L, and adjust the initial pH to 8.0; For protease, add 13000U per gram of dried donkey-hide gelatin, and enzymatically hydrolyze at 55°C for 110 minutes to obtain a hydrolyzate;

[0061] The dried donkey-hide gelatin lump is an intermediate product of the concentration process in the production process of donkey-hide gelatin, with a water content of 13.1% and a protein content of donkey-hide gelatin of 86.8%;

[0062] Described buffer solution is the disodium hydrogen phosphate-sodium hydroxide solution of pH7.5;

[0063] The compound enzyme method is to carry out synchronous enzymolysis after mixing the added alkaline protease and neutral protease with an enzyme activity unit of 1:4;

[0064] Described alkaline protease, neutral protease are food grade;

[0065] The constant temper...

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Abstract

The invention discloses a method for preparing a small molecular gelatin peptide by using gelatin pieces, and belongs to the field of biotechnology. The donkey-hide gelatin peptide is obtained by using dry gelatin pieces as a raw material, separating and purifying gelatin protein by using composite protease enzymolysis, decolorizing by activated carbon, ultra-filtration and spray drying. The preparation method has the advantages of low cost, high enzymolysis degree, short enzymolysis time, less pollution and high yield. The obtained gelatin peptide is directly absorbed by a human body in its functional form, the energy consumption is low, and the absorption rate is high. The function of donkey-hide gelatin is maintained, and the unique physiological function is also achieved.

Description

technical field [0001] The invention relates to a method for preparing small-molecule donkey-hide gelatin peptides by using donkey-hide gelatin lumps, and belongs to the field of biotechnology. Background technique [0002] Ejiao has a long history of development. It can be said that Ejiao is like an evergreen tree. Although it has experienced thousands of years of wind and rain, it has become more and more vigorous. Donkey-hide gelatin was first recorded in "Shen Nong's Materia Medica", more than 2,000 years ago, and has a long history. It is a traditional nourishing and blood-enriching top grade, and is known as the "sacred medicine" for blood-enriching. [0003] Donkey-hide gelatin is a solid glue obtained by decocting and concentrating donkey skin. The main component is collagen, accounting for about 80%. It is difficult to digest and absorb because of its large molecular weight. The small molecule donkey-hide gelatin peptide obtained by enzymatic hydrolysis technology ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06
Inventor 王元秀査士银杨新华
Owner UNIV OF JINAN
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