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Culture system for in-vitro amplification of hematopoietic stem cells

A technology of hematopoietic stem cells and culture system, which is applied in the field of expanding hematopoietic stem cells, and can solve problems such as difficulty in meeting the quantity required for hematopoietic stem cell transplantation, insufficient number of hematopoietic stem cells, limited number of hematopoietic stem cells, etc.

Pending Publication Date: 2018-07-10
LIANGEN BIOLOGICAL SCI & TECH SHANGHAI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Bone marrow and peripheral blood are currently widely used sources of hematopoietic stem cells, but there are problems such as difficulty in matching
Transplantation of hematopoietic stem cells derived from umbilical cord blood can avoid these problems to a certain extent, but the number of hematopoietic stem cells derived from umbilical cord blood is limited, and it is difficult to meet the number required for hematopoietic stem cell transplantation. Therefore, in vitro expansion of hematopoietic stem cells is a solution A key pathway to the problem of insufficient numbers of hematopoietic stem cells in hematological diseases

Method used

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  • Culture system for in-vitro amplification of hematopoietic stem cells
  • Culture system for in-vitro amplification of hematopoietic stem cells
  • Culture system for in-vitro amplification of hematopoietic stem cells

Examples

Experimental program
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Embodiment 1

[0097] Example 1. Expansion of hematopoietic stem cells in vitro

[0098] 1.1 Isolation of monocytes

[0099] Human umbilical cord blood (provided by the Department of Obstetrics and Gynecology, Shanghai First Maternity and Infant Hospital) was collected in a blood bag containing sodium citrate / citric acid / glucose as an anticoagulant, and the blood was stored at 4°C for no more than 48 hours. Take a test tube and add an appropriate volume of cord blood to it, dilute the cord blood with an equal volume of phosphate buffered saline (PBS) supplemented with 2% fetal bovine serum (FBS), then take another test tube and add lymph Cell separation agent, then carefully spread the diluted umbilical cord blood on the lymphocyte separation agent (---purchase source), avoid mixing the lymphocyte separation agent with the diluted umbilical cord blood, and centrifuge at a speed of 800g at room temperature for 20 Minutes to 30 minutes, remove the upper plasma layer after centrifugation wit...

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Abstract

The invention provides a culture system for hematopoietic stem cell amplification. The culture system comprises a basal culture medium and a composition for promoting hematopoietic stem cell amplification, and the composition contains early hematopoietic growth factors related to hematopoietic regulation. The invention further provides a method adopting the culture system for in-vitro amplification of hematopoietic stem cells and a kit comprising the composition for promoting hematopoietic stem cell amplification. By adopting the culture system for in-vitro amplification of the hematopoietic stem cells, the hematopoietic stem cells can be amplified by more than hundreds of times, so that the problem of hematopoietic stem cell insufficiency in the process of treating blood system diseases can be solved effectively.

Description

technical field [0001] The invention relates to the field of stem cell culture, specifically, the invention relates to a culture system for expanding hematopoietic stem cells in vitro and a method for expanding hematopoietic stem cells using the culture system. Background technique [0002] Hematopoietic stem cells have the ability of self-renewal, high reproduction and differentiation in multiple directions, so they have a wide range of applications in the treatment of leukemia, aplastic anemia and other blood system diseases. At present, hematopoietic stem cell transplantation is mainly used to treat blood system diseases. There are three main sources of hematopoietic stem cells for transplantation: bone marrow, peripheral blood and umbilical cord blood. Bone marrow and peripheral blood are currently widely used sources of hematopoietic stem cells, but there are problems such as difficulty in matching. Transplantation of hematopoietic stem cells derived from umbilical cor...

Claims

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Application Information

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IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2501/125C12N2500/90C12N2501/145C12N2501/999
Inventor 张洁曹明喆沈思宇
Owner LIANGEN BIOLOGICAL SCI & TECH SHANGHAI
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