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Tissue culture seedling growing method for Dendrobium signatum

A technology of tissue culture and yellow throat stone, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of hindered breeding of Dendrobium yellow throat seedlings, scarcity of provenance, and few female parents, etc., and achieve high-efficiency tissue culture. Seedling method, the effect of promoting further growth and reducing the cost of cultivation

Inactive Publication Date: 2018-07-10
康美药业(昆明)种质资源有限公司
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  • Claims
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Problems solved by technology

[0003] At present, Dendrobium dendrobii in the domestic market is mainly imported from Myanmar. There is no artificial cultivation, and there are few female parents, and the provenance is scarce; it is generally propagated by division and cuttings, with fewer seedlings and low reproduction rate. Low, long growth cycle, less chance of fruiting without artificial pollination under normal growth environment; due to the constraints of many factors such as few fruit pods, small fruit powder, difficult to germinate and grow in the natural environment, the yellow throat with high ornamental value The breeding of Dendrobium seedlings is hindered

Method used

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  • Tissue culture seedling growing method for Dendrobium signatum

Examples

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Effect test

Embodiment 1

[0039] A method for raising seedlings by tissue culture of dendrobium chrysanthemum, comprising the following steps:

[0040] 1) Selection of explants: Select a healthy 3-year-old female parent plant of Dendrobium japonica, artificially pollinate Dendrobium japonica during the flowering period in late April, and allow it to grow fruit pods. After 3-4 Cultivate in one month, choose the yellow-green outer seed shell, intact and uncracked fruit pods as explants, and store the explants at 3-6°C for 2 weeks;

[0041] 2) Disinfection of explants: get the explants obtained in step 1), soak them with washing liquid, brush the pod epidermis, wash them, soak and disinfect them with medical alcohol under aseptic environment, and then adopt 2wt% sodium hypochlorite successively , 0.1wt% mercury liter disinfection, rinsed;

[0042] 3) Sowing: dissect the explants after step 2) disinfection, take the fruit powder in the fruit pods, sprinkle 0.1g fruit powder on 30mL primary medium, place a...

Embodiment 2

[0047] The difference between embodiment 2 and embodiment 1 is that the 2-year-old dendrobium is selected as the female plant.

Embodiment 3

[0049] The difference between embodiment 3 and embodiment 1 is that in step 3), the light intensity is 2500lx.

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Abstract

The invention discloses a tissue culture seedling growing method for Dendrobium signatum. The method comprises the following steps: explant selection, explant disinfection, seed sowing, subculture, rooting culturing, seedling hardening and domestication transplanting. The method combines the differentiation and growth characteristics of the Dendrobium signatum, allows Dendrobium signatum plants tobe efficiently and economically obtained under appropriate disinfection mode, light intensity, temperature and medium conditions, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for growing seedlings of Dendrobium chrysogenum through tissue culture. Background technique [0002] Dendrobium signatum belongs to Orchidaceae, mainly distributed in Myanmar, and less distributed in China, most of which are imported from Myanmar; the stem of Dendrobium signatum is drooping, slightly fleshy, cylindrical, unbranched, with many nodes. Multiple racemes, plant height, and deciduous old stems, each flower stalk usually has 2 flowers, the flowers are large, white, membranous, and spread out; the petals are nearly oval, the lips are oval and white, and the sides are surrounded The embracing column forms a trumpet shape, and the trumpet-shaped part is yellow. Yellow Throat resources are scarce, with large flowers, elegant colors, and a flowering period of up to 3 months, which has high ornamental value. [0003] At present, Dendrobium dendrobii in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许冬瑾严新顾晓波杜永魏
Owner 康美药业(昆明)种质资源有限公司
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