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A kind of tissue culture seedling raising method of Dendrobium primula

A tissue culture, primrose technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of reduced resources of Dendrobium primula, difficult to germinate and grow, long growth cycle, etc., to promote further growth, The effect of reducing the cost of cultivation and promoting the rooting of seedlings

Active Publication Date: 2021-04-16
康美药业(昆明)种质资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Dendrobium primula in the domestic market is mainly imported and wildly excavated. There are a few artificial cultivations, which are generally propagated by division and cuttings. There are few seedlings for propagation, low reproduction rate, long growth cycle, and normal growth. The probability of fruiting without artificial pollination in the environment is small; due to the constraints of many factors such as few fruit pods, small fruit powder, and difficult germination and growth in the natural environment, the wild resources of Dendrobium primula are constantly decreasing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for raising seedlings by tissue culture of Dendrobium primula, comprising the following steps:

[0039] 1) Selection of explants: select a healthy 3-year-old female parent plant of Dendrobium primula, artificially pollinate Dendrobium primula during the flowering period in late March, and allow it to grow fruit pods. After 3-4 Cultivate in one month, choose the yellow-green outer seed shell, intact and uncracked fruit pods as explants, and store the explants at 3-6°C for 2 weeks;

[0040] 2) Disinfection of explant: get the explant that step 1) obtains, soak with washing liquid, brush pod epidermis, clean, soak and disinfect with medical alcohol under aseptic environment, then adopt the sodium hypochlorite of 2wt% successively , 0.1wt% mercury liter disinfection, rinsed;

[0041] 3) Sowing: Dissect the explants after step 2) disinfection, take the fruit powder in the fruit pods, sprinkle 0.1g fruit powder on 30mL primary culture medium, place at 25°C, light in...

Embodiment 2

[0046] The difference between embodiment 2 and embodiment 1 is that a 2-year-old Dendrobium primula is selected as the female parent plant.

Embodiment 3

[0048] The difference between embodiment 3 and embodiment 1 is that in step 3), the light intensity is 2500 lx.

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Abstract

The invention discloses a method for raising dendrobium dendrobium seedlings by tissue culture, which comprises the following steps: selection of explants, disinfection of the explants, sowing, subculture, rooting culture, hardening of seedlings and domestication and transplanting. Combining the differentiation and growth characteristics of Dendrobium primula, this method selects the appropriate disinfection method, light intensity, temperature and medium, and obtains Dendrobium primula plants in an efficient and economical way, which has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for growing seedlings of Dendrobium primula by tissue culture. Background technique [0002] Dendrobium signatum Lindl. is an orchid family, mainly distributed in India, Myanmar, Thailand, Laos, Vietnam and other countries. Stem drooping, thick fleshy, cylindrical, unbranched, with many nodes; raceme with 1-3 flowers, usually from stem nodes with fallen leaves, flowers drooping, sepals and petals light rose, lips Petals light yellow with light rose apex, densely pubescent on both sides, with irregular serrations on the edge, lip disc with purple-red veins, flowering period March-April. [0003] At present, Dendrobium primula in the domestic market is mainly imported and wildly excavated. There are a few artificial cultivations, which are generally propagated by division and cuttings. There are few seedlings for reproduction, low reproduction rate, long growth cyc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 许冬瑾严新雷连海杜永魏
Owner 康美药业(昆明)种质资源有限公司
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