Image-based white blood cell counting method and system
A white blood cell counting and white blood cell technology, which is applied in the field of white blood cell counting systems, can solve the problems of inability to obtain cell segmentation, low counting accuracy, and difficulty in distinguishing concave and convex points on the boundary.
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[0090] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0091] Please refer to figure 1 Referring to FIG. 7 , the present invention proposes an image-based white blood cell counting method for fast, accurate and convenient counting of the number of white blood cells in human peripheral blood. The specific steps are as follows.
[0092] Step S1: Image Acquisition
[0093] In order to improve the counting accuracy of white blood cells, before the method is implemented, trypan blue is used to stain the white blood cells in the blood sample to enhance the contrast of the cells, and then the red blood cells in the blood sample are lysed with the red blood cell lysate to reduce the interference of irrelevant cell impurities. The leukocyte staining and erythrocyte lysing methods in this step can also be reali...
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