Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An image-based white blood cell counting method and system

A white blood cell counting and white blood cell technology, which is applied in the field of white blood cell counting systems, can solve the problems of inability to distinguish the nucleus area, inability to identify adherent cells, and difficulty in distinguishing the concave and convex points on the boundary.

Active Publication Date: 2020-08-25
JIANGSU KONSUNG BIOMEDICAL TECH
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this case, the nucleus area cannot be distinguished on the image, and it is difficult to construct a cell machine model; under a 4-20 magnification microscope, the white blood cells have blurred boundaries and a large number, and the method based on morphological boundary extraction still has low precision. When the adhesion cells cannot be identified, the iterative convergence of the B-snake model is too slow and takes a long time; the boundary of the adhesion cells is blurred, and it is difficult to distinguish the concave and convex points of the boundary, and the discrimination error is large
To sum up, the application of the above methods cannot obtain accurate cell segmentation, cannot completely separate the adherent cells, and there is a problem of low counting accuracy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An image-based white blood cell counting method and system
  • An image-based white blood cell counting method and system
  • An image-based white blood cell counting method and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0090] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0091] Please refer to figure 1 Referring to FIG. 7 , the present invention proposes an image-based white blood cell counting method for fast, accurate and convenient counting of the number of white blood cells in human peripheral blood. The specific steps are as follows.

[0092] Step S1: Image Acquisition

[0093] In order to improve the counting accuracy of white blood cells, before the method is implemented, trypan blue is used to stain the white blood cells in the blood sample to enhance the contrast of the cells, and then the red blood cells in the blood sample are lysed with the red blood cell lysate to reduce the interference of irrelevant cell impurities. The leukocyte staining and erythrocyte lysing methods in this step can also be reali...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an image-based white blood cell counting method and system, wherein the method includes the steps of collecting blood sample images, removing image noise, roughly segmenting cell regions, obtaining the number of cohesive cells and obtaining the total number of white blood cells. Because the present invention adopts the white blood cell automatic segmentation technology of self-adaptive fuzzy clustering technology, the white blood cell segmentation accuracy is higher, and the adhesion cell location segmentation method based on the three-dimensional curved surface can quickly identify the adhesion cells with inconspicuous area and chain code characteristics, adhesion cells The location counting segmentation is more accurate, so that the number of white blood cells can be counted more accurately.

Description

technical field [0001] The invention relates to an image-based white blood cell counting method, and at the same time, the invention also relates to a white blood cell counting system. Background technique [0002] As an indication of certain diseases, the number of white blood cells has important clinical significance for disease diagnosis. Physical method, physical-chemical method and image analysis method are the main methods of white blood cell differential counter at present. Although the automatic counters under these methods can quickly and accurately count white blood cells, they still have the characteristics of bulky equipment, high price, high professionalism in operation, and difficult maintenance, which are not suitable for primary medical and health institutions and mobile medical services. However, there are great limitations in the field of application. For the image-based leukocyte adhesion segmentation and counting method, it mainly focuses on the researc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G06T7/00G06T7/11G06T7/13G06T5/00G06K9/62
CPCG06T5/002G06T7/0012G06T7/11G06T7/13G06T2207/10056G06T2207/20004G06T2207/30242G06T2207/30004G06F18/23
Inventor 张雪项智李迎春
Owner JIANGSU KONSUNG BIOMEDICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products