Multiple liquid chip for detecting tuberculosis antibody

Abstract

The invention discloses a multiple liquid chip for detecting a tuberculosis antibody. The chip comprises antigen coupled coded microspheres and a biotinylated antibody, wherein the ntigen coupled coded microspheres are obtained by coupling LAM, CFP-10, EAST-6, 16KD, 38KD, 65KD and a TBLG antigen, CFP10-EAST6 and 38KD-16KD fusion protein, chicken IgG, Biotin-BSA, BSA and SA-PE to different coded fluorescent microspheres separately; the biotinylated antibody is obtained by marking rabbit-anti-chicken IGg and goat-anti-human IgG with carboxyl active biotin. A 65KD, CFP10-EAST6 and 38KD-16KD multiple detection system is established. The result shows that the multiple system can differentiate and make a definite diagnosis on patients with tuberculosis and patients without tuberculosis (P is smaller than 0.05) effectively, the positive rate is 88.73% which is obviously higher than the detection results of four kits: T-SPOT, TB-DOT, TB-CHECK-1 and Syptak (51.2%, 50.2%, 39.4% and 65.7%). Halfyear clinical experiments verify that the system is good in stability, good in specificity, high in sensitivity, good in repeatability and quick in detection time, and the advantages of the established multiple detection system established are initially displayed.

Description

technical field [0001] The invention relates to a liquid-phase diagnostic chip, in particular to a multiple liquid-phase chip for detecting tuberculosis antibodies. Background technique [0002] The development of the global economy, the opening of trade, and the convenience of transportation have all opened the door for travel, family visits, and transactions in various countries. The speed of population mobility has accelerated, and at the same time, the spread of infectious diseases has also spread. [2] . In recent years, tuberculosis has resurged worldwide, seriously threatening human health, and has become one of the most serious public health problems. Infectious tuberculosis is currently the only disease that is clearly prohibited from entering the country in the "Rules for the Implementation of the Frontier Health and Quarantine Law of the People's Republic of China". The survey shows that one of the main reasons for the obvious rebound of the tuberculosis epidemic...

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Problems solved by technology

[0004] In the past two decades, many scholars have devoted themselves to using immunological methods to detect tuberculosis serum antibodies as an auxiliary means of tuberculosis screening. Studies have proved that this method is feasible and greatly improved the sensitivity of smears, X-rays and other methods , our research group has also screened common tuberculosis antigens in the past ten years, and identified several antigens with high sensitivity, but previous studies have shown that when different antigens are mixed in an ELISA plate, The content and interaction of different antigens will affect the accuracy of the test results

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