Multispectral fluorescence segmentation method and apparatus

Abstract

Embodiments of the invention provide a multispectral fluorescence segmentation method and apparatus. The method comprises the steps of obtaining a multispectral fluorescence segmentation neural network, wherein the multispectral fluorescence segmentation network is obtained by training a three-dimensional convolutional neural network; taking multiple groups of surface excitation fluorescence images of different wavelengths obtained under a laser excitation condition as input data required for training, and taking multiple groups of surface fluorescence images of different wavelengths obtainedunder no laser excitation condition as a fluorescence spot distribution result of training; and obtaining to-be-processed multispectral excitation fluorescence images, and inputting the to-be-processed multispectral excitation fluorescence images to the multispectral fluorescence segmentation neural network, thereby obtaining a segmentation result of excitation fluorescence spots. According to thescheme, the situation that the fluorescence images of the different fluorescence wavelengths have optical correlation, so that an effective fluorescence region can be accurately divided and the accuracy of the segmentation result of the excitation fluorescence spots can be improved.

Description

technical field [0001] The invention relates to the technical field of optical imaging, in particular to a multispectral fluorescence segmentation method and device. Background technique [0002] Optical imaging technology is an emerging molecular imaging technology in recent years. It has the advantages of high sensitivity, non-ionizing radiation, and simple operation. It is a technology that can realize biophysical process imaging inside biological tissues at the molecular level. In addition, optical imaging technology is being gradually applied to clinical surgical navigation and other fields by mounting fluorescence cameras and laser light sources on endoscopes. By injecting the corresponding targeted probe drug into the imaging object, and irradiating the observation site with the laser mounted on the endoscope, the fluorescence information of the observation site under the laser irradiation is captured. However, due to the influence of the excitation light source and ...

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Problems solved by technology

[0003] An embodiment of the present invention provides a multi-spectral fluorescence segmentation method to solve the technical problem of low accuracy in the segmentation results of excited fluorescence spots in the prior art

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