Nutrient slow-release filler loaded with functional microbes and application of filler
A microbial and functional technology used in the field of biological purification of environmental pollution
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Embodiment 1
[0038] Embodiment 1: packing support preparation process
[0039] (1) Filler carrier
[0040] At room temperature (25-30°C), slowly add 9g of sodium alginate powder into 600mL of inorganic salt culture solution under high-speed stirring at 160rpm, and continue stirring for 2 hours to obtain a colorless and transparent 15g / L sodium alginate solution mixed with inorganic nutrients. Then 50mL of sodium alginate solution was divided into 100mL beakers, and placed in a 4°C freezer for 16h until it became viscous. Spray 0.2mol·L after taking it out -1 CaCl 2 The aqueous solution a forms a film on its surface ( Figure 4 middle a), and then slowly add 0.2mol L by glass rod drainage -1 CaCl 2 Aqueous solution b, CaCl 2 Aqueous solution a and CaCl 2 The total volume of the aqueous solution b was 20mL, and the alginate gel was obtained after standing at 4°C for 72 hours, and finally freeze-dried at -81°C for 60 hours to form a porous structure and obtain a filler scaffold with a ...
Embodiment 2
[0046] Example 2: CaCl 2 Join method optimization
[0047] The preparation method of conventional calcium alginate gel is to adopt one-step method, promptly under normal temperature or low temperature 50ml sodium alginate solution (same as embodiment 1) directly mixes 20mL, 0.2mol L -1 CaCl 2 The aqueous solution was mixed, and the alginate gel was obtained after standing at 4°C for freezing reaction for 72 hours, and finally freeze-dried at -81°C for 60 hours to prepare a calcium alginate gel with a porous structure. However, the gel prepared by this method is often porous, which is not suitable for the transfer of nutrients and the growth of microorganisms.
Embodiment 3
[0048] Example 3: Evaluation of slow release performance of filler carrier with nutrient slow release function
[0049] (1) Filler carrier
[0050] The filler support with nutrient slow release function is prepared by the principle of emulsification and crosslinking. The preparation process is as follows: at room temperature, slowly add 18g of sodium alginate powder to 600mL of inorganic salt culture medium under high-speed stirring at 160rpm, and continue stirring for 2 hours to obtain a colorless Transparent 30g / L sodium alginate solution mixed with inorganic nutrients. Then 50mL sodium alginate solution was divided into 100mL beakers, and placed in a 4°C freezer for 24h until it became viscous. After taking it out, spray 0.1mol L on its surface -1 CaCl 2 Aqueous solution a forms a thin film, and then slowly add 0.1mol L -1 CaCl 2 Aqueous solution b, CaCl 2 Aqueous solution a and CaCl 2 The total volume of the aqueous solution b was 25 mL, and the alginate gel was obt...
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