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Preparation method and application method of core-shell nanocrystal cell temperature sensor

A temperature sensor, core-shell nanotechnology, applied in the field of biosensing, to achieve the effects of low cytotoxicity, improved luminous efficiency, and good reproducibility

Active Publication Date: 2018-06-22
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NaYF used by researchers 4 :Er 3+ , Yb 3+ As an intracellular temperature measurement, the resolution can reach 0.5K, but it is far below the urgent demand for high-resolution intracellular temperature measurement (0.01K) in the future

Method used

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  • Preparation method and application method of core-shell nanocrystal cell temperature sensor
  • Preparation method and application method of core-shell nanocrystal cell temperature sensor
  • Preparation method and application method of core-shell nanocrystal cell temperature sensor

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Embodiment 1

[0035] 1. A method for preparing a core-shell nanocrystalline cell temperature sensor, the steps are as follows:

[0036] 1) Preparation of spherical NaYF with uniform size by thermal decomposition method 4 :Yb 3+ ,Er 3+ core nanocrystals, and then use the same method to wrap NaYF on the core nanocrystals 4 :Yb 3+ ,Nd 3+ The shell gets rare earth doped NaYF 4 Core-shell nanocrystals, the diameter of the core is 20-30nm, and the thickness of the shell is 10-15nm;

[0037] 2) Dissolve 2 mmol of the obtained core-shell nanocrystals in 2 ml of toluene by a traditional method, stir at room temperature for 3 hours at a stirring frequency of 500 rpm, and add 2 ml of dimethyl sulfide that dissolves 30 mg of dimercaptosuccinic acid (DMSA). In the sulfone (DMSO) solution, under the condition of a stirring frequency of 500rpm, magnetically stirred for 12 hours; the above sample was purified by dialysis for 3 days, and the pH was adjusted to 10.5 to obtain the hydrophilic phase NaYF...

Embodiment 2

[0042] 1. A method for preparing a core-shell nanocrystalline cell temperature sensor, the steps are as follows:

[0043] 1) Preparation of spherical NaYF with uniform size by thermal decomposition method 4 :Yb 3+ ,Er 3+ core nanocrystals, and then use the same method to wrap NaYF on the core nanocrystals 4 :Yb 3+ ,Nd 3+ The shell gets rare earth doped NaYF 4 Core-shell nanocrystals, the diameter of the core is 20-30nm, and the thickness of the shell is 10-15nm;

[0044] 2) Dissolve 4 mmol of the obtained core-shell nanocrystals in 2 ml of toluene by a traditional method, stir at room temperature for 5 hours at a stirring frequency of 1000 rpm, and add 2 ml of dimethyl sulfide dissolved in 40 mg of dimercaptosuccinic acid (DMSA). In the sulfone (DMSO) solution, under the condition of stirring frequency of 1000rpm, magnetically stirred for 10 hours; the above sample was dialyzed and purified for 2 days, and the pH was adjusted to 10 to obtain the hydrophilic phase NaYF 4...

Embodiment 3

[0049] 1. A method for preparing a core-shell nanocrystalline cell temperature sensor, the steps are as follows:

[0050] 1) Prepare spherical NaYF with uniform size by co-precipitation method 4 :Yb 3+ ,Er 3+ core nanocrystals, and then use the same method to wrap NaYF on the core nanocrystals 4 :Yb 3+ ,Nd 3+ The shell gets rare earth doped NaYF 4 Core-shell nanocrystals, the diameter of the core is 20-30nm, and the thickness of the shell is 10-15nm;

[0051] 2) Dissolve 2 mmol of the obtained core-shell nanocrystals in 2 ml of toluene by a traditional method, stir at room temperature for 4 hours at a stirring frequency of 700 rpm, and add 10 ml of dimethyl sulfide dissolved in 10 mg of dimercaptosuccinic acid (DMSA). In the sulfone (DMSO) solution, under the condition of a stirring frequency of 800rpm, magnetically stirred for 15 hours; the above sample was purified by dialysis for 4 days, and the pH was adjusted to 11 to obtain the hydrophilic phase NaYF 4 :Yb 3+ ,Er...

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Abstract

The invention discloses a preparation method and an application method of a core-shell nanocrystal cell temperature sensor. The core-shell nanocrystal cell temperature sensor is a core-shell nanocrystal cell temperature sensor based on rare earth-doped NaYF4. The preparation steps are as follows: rare earth-doped NaYF4 core-shell nanocrystals are first prepared, and are prepared into a solution after hydrophilization, cells are cultured in the solution, and thereby the core-shell nanocrystal cell temperature sensor is obtained. When the sensor is applied, laser is utilized to activate marked to-be-measured cells, a temperature-dependent upconversion fluorescence spectrum of the marked to-be-measured cells is measured, and a functional relation between luminous intensity and cell temperature is established. By ingeniously designing the core-shell structure nanocrystals, the invention gets temperature sensing characteristics deep into the cellular level, consequently, the resolution of cell temperature measurement is greatly increased, the repeatability is good, and cytotoxicity is low.

Description

technical field [0001] The invention relates to a preparation method and an application method of a core-shell nanocrystal cell temperature sensor, belonging to the field of biosensing. Background technique [0002] Accurate measurement of intracellular temperature is essential for a clear understanding of complex biological processes. In 2012, Okabe et al. invented the first intracellular temperature measurement based on fluorescent polymer thermometer and fluorescence lifetime imaging microscopy, revealing the intrinsic link between temperature and organelle function. Optical thermometry, as a non-contact temperature sensing method, has attracted widespread attention due to its inviolability, fast response, and high sensitivity, making it suitable for environments with harsh electromagnetic and thermal environments. There is a relationship between the spectrum of certain ions in the rare earth luminescence and the external temperature. Taking advantage of the excellent lu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/85B82Y30/00B82Y40/00G01K11/32C12Q1/02
CPCG01K11/32G01N33/5005C09K11/025C09K11/7773B82Y30/00B82Y40/00
Inventor 石增良段越徐春祥
Owner SOUTHEAST UNIV
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