Method for testing applicability of plant to karst environments

A karst and botanical technology, applied in botany equipment and methods, testing plants/trees, applications, etc., can solve the problems of cumbersome physiological and biochemical indicators

Inactive Publication Date: 2018-06-19
GUIZHOU NORMAL UNIVERSITY +1
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for measuring the ability of plants to adapt to the karst environment, so as to overcome the shortcomings of previous techniques such as the cumbersome determination of related plant physiological and biochemical indicators, and solve the problem of simple and quick accurate measurement of plants in various adversities. The conundrum of plant adaptability to different karst environments

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  • Method for testing applicability of plant to karst environments
  • Method for testing applicability of plant to karst environments
  • Method for testing applicability of plant to karst environments

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[0016] It includes the following steps: First, the same specifications of plugs are used indoors to germinate plant seeds, prepare Hoagland culture medium to cultivate seedlings to 1 month old, select healthy seedlings with more consistent growth and divide them into two parts, one part is used for determination Some of the photosynthesis and chlorophyll fluorescence indicators of plants are used to determine the index of root-shoot ratio; secondly, by adding or reducing chemical substances to the Hoagland nutrient solution, in order to simulate karst stress factors indoors: karst drought (adding PEG, Simulate osmotic stress), high bicarbonate (add NaHCO 3 , To simulate HCO3-stress), low nutrition (to reduce the overall concentration of Hoagland nutrient components), phosphorus deficiency (only reduce the concentration of phosphorus in Hoagland), partial alkali (to adjust the pH of Hoagland nutrient solution to 8.3), among which Hoagland The plant seedlings cultivated in blue nu...

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Abstract

The invention discloses a method for testing applicability of plants to a karst environment. The method is characterized by comprising the following steps: adding or reducing chemical substances froma Hogland nutrient liquid, and simulating karst adverse factors indoor, wherein plant seedlings cultured by using the Hogland nutrient liquid are adopted for reference treatment; respectively testingnet photosynthetic rates, PSII (Plasma Source Ion Implantation) maximum photochemical efficiency and root shoot ratio indexes of tested plants on the tenth day and the twentieth day; respectively comparing the net photosynthetic rates, the PSII maximum photochemical efficiency and the root shoot ratio indexes acquired from reverse factor culture plants with those of the reference, normalizing theindexes, and respectively calculating the degradation amplitudes a, b and c of the net photosynthetic rates, the PSII maximum photochemical efficiency and the root shoot ratio indexes of the plants under reverse factors; according to the degradation amplitudes a, b and c of the net photosynthetic rates, the PSII maximum photochemical efficiency and the root shoot ratio indexes of the plants underthe reverse factors, judging and observing applicability of the plants to reverse environments.

Description

Technical field [0001] The invention relates to a method for determining the ability of plants to adapt to the karst environment, and belongs to the field of ecological environment system monitoring, treatment and restoration. Background technique [0002] my country's karst area is widely distributed, and the ecological environment is heterogeneous, diverse and fragile. Many scholars in related fields have found that karst environment has many negative influence factors on plants, and the degree of influence varies. Due to the interference of geological processes and unreasonable man-made activities, the fragile karst areas are partially exposed, the soil is shallow and barren, and the water retention capacity is poor, the vegetation coverage rate is low, and the ecological environment is facing a deteriorating trend. This has a limited cultivation area. The Karst area is undoubtedly worse. In response to the urgent need for treatment and restoration of the karst environment, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00A01G31/00
CPCA01G31/00G01N33/0098
Inventor 杭红涛吴沿友熊康宁张开艳李海涛谭代军陆娜娜闵小莹郭涛
Owner GUIZHOU NORMAL UNIVERSITY
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