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A method for determining tea 1,2,6 trigalloyl-β-d-glucose and its application

A technology of galloyl and glucose, applied in the field of determination of tea 1

Active Publication Date: 2020-12-08
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no standard product of this substance on the market at present, and the content of 1,2,6 trigalloyl-β-D-glucose in most plants is also very low. How to discover and utilize this substance is a major problem in the agricultural and pharmaceutical industries

Method used

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  • A method for determining tea 1,2,6 trigalloyl-β-d-glucose and its application
  • A method for determining tea 1,2,6 trigalloyl-β-d-glucose and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Tea sample preparation: Pick the tea samples to be tested (one bud and one leaf or one bud and two leaves) during the spring tea season, make dry tea and grind it into tea powder with a sample mill.

[0023] 2) Sample extraction: Accurately weigh 0.2 g of tea sample, add 5 mL of extraction solution (70% methanol solution), extract in a water bath at 70°C for 10 minutes (shake every 5 minutes), cool to room temperature, transfer to a centrifuge at 3500r Centrifuge at a speed of 10 min / min, take out the supernatant, and then add 5ml of extract to extract once, combine the extract to 10 mL, and shake well. Pipette 2 mL of the extract into a 10 mL graduated tube, add 8 mL of stabilizing solution to constant volume, pass through a 0.45 μm organic microporous membrane, and take the filtrate for subsequent determination.

[0024] 3) Sample determination: UPLC-Q-TOF-MS / MS ultra-high pressure liquid chromatography-time-of-flight mass spectrometer was used to determine the con...

Embodiment 2

[0026] 1) Preparation of tea samples: pick the tea samples to be tested in the spring tea season, make dry tea and grind them into tea powder with a mill;

[0027] 2) Sample extraction: Accurately weigh 0.15 g of tea sample, add 4 mL of 65% methanol solution, extract in a 65°C water bath for 9 min, shake once every 5 min, cool to room temperature, transfer to a centrifuge at 3200r / min Centrifuge for 9 minutes, take out the supernatant, add 4ml of 65% methanol solution to extract once, combine the extracts to 10 mL, shake well, draw 2 mL of extracts into a 10 mL graduated tube, add 8 mL of stable solution to volume, Pass through a 0.45 μm organic microporous membrane, and take the filtrate for subsequent determination;

[0028] 3) Determination of samples: Determination of 1, 2, 6 trigalloyl-β-D-glucose content using ultra-high pressure liquid chromatography-time-of-flight mass spectrometer;

[0029] Mobile phase: 0.1% formic acid aqueous solution (A) - acetonitrile (B) contai...

Embodiment 3

[0033]1) Preparation of tea samples: pick the tea samples to be tested in the spring tea season, make dry tea and grind them into tea powder with a mill;

[0034] 2) Sample extraction: Accurately weigh 0.25 g of tea sample, add 6 mL of 70% methanol solution, extract in 75°C water bath for 11 min, shake once every 5 min, cool to room temperature, transfer to centrifuge at 3600r / min Centrifuge for 11 min, take out the supernatant, add 6ml of 65% methanol solution to extract once, combine the extracts to 10 mL, shake well, draw 2 mL of extracts into a 10 mL graduated tube, add 8 mL of stable solution to volume, Pass through a 0.45 μm organic microporous membrane, and take the filtrate for subsequent determination;

[0035] 3) Determination of samples: Determination of 1, 2, 6 trigalloyl-β-D-glucose content using ultra-high pressure liquid chromatography-time-of-flight mass spectrometer;

[0036] Mobile phase: 0.1% formic acid aqueous solution (A) - acetonitrile (B) containing 0....

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Abstract

The invention provides a determination method of 1,2,6-tri-galloyl-beta-D-glucose in tea and an application of the determination method, and belongs to the technical field of tea detection methods. The method comprises the following steps: 1) preparation of a tea sample; 2) sample extraction; 3) sample determination: the content of 1,2,6-tri-galloyl-beta-D-glucose is determined by an ultrahigh-pressure liquid chromatography-time-of-flight mass spectrometer. According to the determination method of 1,2,6-tri-galloyl-beta-D-glucose in the tea and the application in tea variety breeding, characteristics of accuracy and high efficiency are achieved, the breeding progress of related varieties can be greatly accelerated, and the determination method is also an ideal method for studying the synthesis mechanism of 1,2,6-tri-galloyl-beta-D-glucose.

Description

technical field [0001] The invention belongs to the technical field of tea detection methods, in particular to a method for measuring tea 1,2,6-trigalloyl-β-D-glucose and its application. Background technique [0002] 1,2,6 Trigalloyl-β-D-glucose is an important class of polyphenolic compounds. Existing studies have shown that the substance has the functions of anti-liver cancer, tumor suppression, inhibition of fusion between HIV and target cells, and anti-multi-drug-resistant bacteria. However, there is no standard product of this substance on the market at present, and the content of 1,2,6 trigalloyl-β-D-glucose in most plants is also very low. How to discover and utilize this substance is a major problem in the agricultural and pharmaceutical industries. Tea leaves are rich in polyphenols, but the content of 1, 2, 6 trigalloyl-β-D-glucose in general varieties is very low, and the variation range is between 0-6.6 mg / g. Therefore, how to detect the content of 1,2,6 triga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 韦康成浩王丽鸳阮丽李海琳吴立赟张亚真
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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