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Method for identifying capparis spinosa and capparis tenera and application thereof

A technology of capers and capers, which is applied in the field of molecular biology detection, can solve problems affecting the quality and safety of medicines, confusion between capers and capers, and poor practicability, and achieve easy mastery and accuracy High and wide applicability

Inactive Publication Date: 2018-06-15
XINJIANG INST OF MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Capparis spinosa L. is a plant of the genus Capparis, also known as wild watermelon, mouse melon, melon, mallet fruit vine, etc. Its fruits and roots are Uighurs and Kazakhs in Xinjiang. and other traditional folk medicinal materials, which have a history of thousands of years and are mainly used to treat rheumatism, arthritis, osteoarthritis and other diseases. They are only distributed in the arid areas of Northwest China, with Xinjiang as the main production area, and all medicinal materials come from wild; Capparis tenera Dalz. is a poisonous plant belonging to the genus Capparis tenera Dalz., which has neurotoxicity. Although capparis tenera Dalz. and Capparis tenera are different in the content of medicinal ingredients, their pharmacological effects are also different. , but their shapes are very similar, it is difficult to distinguish them by traditional methods, and the practicability is not strong. The discriminator needs to have certain professional background knowledge and rich experience, which leads to the confusion of capers and capers in the market. To a certain extent, it affects the quality and safety of medication

Method used

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  • Method for identifying capparis spinosa and capparis tenera and application thereof
  • Method for identifying capparis spinosa and capparis tenera and application thereof
  • Method for identifying capparis spinosa and capparis tenera and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Unless otherwise specified, the following examples are all in accordance with conventional experimental conditions, such as the operating technical regulations described in the molecular cloning experimental manual such as Sambrook (New York: Gold Spring Harbor Laboratory Press, 1989), or according to the experimental conditions suggested by the manufacturer ; The raw materials used are all commercially available commodities. Example 1 Establishment of the identification method of capers and capers

[0033] 1.1 Sample source

[0034] The sample sources of capers and capers used in this example are shown in Table 1:

[0035] Table 1 Sample sources of capers and capers

[0036]

[0037] 1.2 DNA extraction

[0038] Take about 30 mg of each sample, quickly grind it into fine powder in liquid nitrogen, and use a plant genomic DNA extraction kit to extract the total DNA of the sample. The specific steps are carried out according to the instructions provided by the kit. ...

Embodiment 2

[0052] Practical application of the identification method of embodiment 2 capers and capers

[0053] 1.1 Sample source

[0054] In this example, the samples of the genus Caper were collected from Turpan, Aksu, Urumqi, Xinjiang; medicinal material markets, such as Xishuangbanna, Yunnan, botanical gardens, or the wild. See Table 2 for details.

[0055] Table 2 Sample sources of Caper

[0056]

[0057] 1.2 DNA extraction

[0058] Take about 30 mg of each sample, quickly grind it into fine powder in liquid nitrogen, and use a plant genomic DNA extraction kit to extract the total DNA of the sample. The specific steps are carried out according to the instructions provided by the kit. The kit uses plant genomic DNA from Beijing Tiangen Company. extraction kit.

[0059] 1.3 PCR primers

[0060] Primers were synthesized by Beijing Huada Gene Co., Ltd. (Beijing). The primers were dissolved with sterile deionization and diluted to 5 μmol / L. The specific primers are as follows:

...

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Abstract

The invention discloses a method for identifying capparis spinosa and capparis tenera and the application thereof. The method comprises the following steps: performing PCR amplification on a psbA-trnHnucleotide sequence of chloroplast DNA, and constructing a phylogenetic tree of the psbA-trnH nucleotide sequence. By the method for identifying the capparis spinosa and the capparis tenera, providedby the invention, the capparis spinosa and the capparis tenera can be identified rapidly and accurately, and the problems of variety identification and improvement of the medicinal capparis spinosa,development and utilization of a germplasm resource and the like are effectively solved; the method is wide in applicability, easy to operate, easy to master and high in accuracy; by the method, rapidand accurate identification of the medicinal materials and powders of the capparis spinosa and the capparis tenera can be successfully achieved.

Description

technical field [0001] The invention relates to the field of molecular biology detection for identifying natural plant medicinal materials, in particular to a method for identifying capers and capers and applications thereof. Background technique [0002] Capparis spinosa L. is a plant of the family Caperaceae, also known as wild watermelon, rat melon, melon, and mallet vine. Its fruits and roots are Uygur and Kazakh in Xinjiang. It is mainly used to treat rheumatism, arthritis, osteoarthritis and other diseases. It is only distributed in the arid areas of the northwest, mainly in Xinjiang, and the medicinal materials are all from the wild; Capparis tenera Dalz. is a poisonous plant belonging to the genus Cauliaceae, and it is neurotoxic. Although Capparis tenera Dalz. is different in the content of medicinal components, its pharmacological effects are also different. , but their shapes are very similar, it is difficult to distinguish them by traditional methods, and their ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806G06F19/24
CPCC12Q1/6806G16B40/00C12Q2531/113
Inventor 程波杨伟俊何江刘冲满尔哈巴·海如拉地力努尔·吐尔逊江徐荣
Owner XINJIANG INST OF MATERIA MEDICA
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