Application of phosphatidylserine (PS) blocking agent to preparation of medicines for treating platelet number reduction related diseases
A technology for phosphatidylserine and thrombocytopenia, applied in the field of platelet-related drugs, can solve problems such as shortening the lifespan of platelets, and achieve the effect of great scientific research and economic value
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[0032] 1. Reagents and materials:
[0033] JC-1 was purchased from Biyuntian Company in China; FITC-human CD62P, PE-human CD41 and PE-mouse CD41 were purchased from American Biolegend Company; R300, FITC-mouse CD62P and PE-JON / A were purchased from German Emfret Company; FITC- PAC1 was purchased from BD Biosciences, USA; anti-Human-caspase-3 antibody was purchased from Biolegend, USA; Fermentas Protein Marker (10-170KD) was purchased from Thermo; FITC-lactadherin was purchased from Haematologic Technologies, USA; Normal Mouse IgG and Normal rat IgG were purchased from from Santa Cruz, USA; AN51 and SZ2 were provided by the Blood Research Institute of the First Affiliated Hospital of Soochow University.
[0034] 2. Experimental mice:
[0035] Wild type (WT) C57BL / 6 mice were purchased from Shanghai Slack Experimental Animal Co., Ltd. All experimental animal-related welfare is strictly implemented in accordance with the "Regulations on the Administration of Experimental Animal...
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