Preparation method of organic selenium enzyme preparation with high conversion rate

A high conversion rate, organic selenium technology, applied in the direction of animal feed, additional food elements, animal feed, etc., can solve the problems of low conversion rate and affecting the use effect.

Inactive Publication Date: 2018-06-12
郑州助协生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this preparation method usually only uses one kind of bacterium to carry out one conversion, and the

Method used

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Examples

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Effect test

Embodiment 1

[0011] A method for preparing an organic selenium enzyme preparation with a high conversion rate, the method is as follows: 81 parts by weight of bran, 13 parts by weight of soybean meal, 0.9 part by weight of rice husk powder, 0.6 part by weight of ammonium sulfate, and 0.4 parts by weight of potassium dihydrogen phosphate 4 parts by weight of malt, 4 parts by weight of malt, the proportioning of 110 parts by weight of sodium selenite solution with a concentration of 0.1% by mixing the above-mentioned materials to obtain a mixture, after stirring evenly, when the water content of the mixture is not enough, use sterile water to Add water to the mixture so that its water content is 65% by weight. Then, under the condition of 0.18MPa and 125°C, the pressure is 0.18MPa and the temperature is 125°C. Carry out high-temperature and high-pressure sterilization for 43 minutes. After the sterilization, the above-mentioned materials are cooled to At 38°C, inoculate 2.3% of its weight of ...

Embodiment 2

[0013] A preparation method of an organic selenium enzyme preparation with a high conversion rate, the method is as follows: 85 parts by weight of bran, 11 parts by weight of soybean meal, 1.1 parts by weight of rice husk powder, 0.4 parts by weight of ammonium sulfate, and 0.6 parts by weight of potassium dihydrogen phosphate 2 parts by weight of malt, 2 parts by weight of malt, the proportioning of 90 parts by weight of sodium selenite solution with a mass percent concentration of 0.2%, the above-mentioned materials are mixed to obtain a compound, after stirring, when the water content of the compound is not enough, use sterile water to Add water to the mixture so that its water content is 55% by weight. Then, under the condition of 0.22MPa and 115°C in pressure, carry out high temperature and high pressure sterilization for 46 minutes. After the sterilization, the above materials are cooled to At 36°C, inoculate 2.7% of its weight of Candidautilis (Candidautilis) aspergillus...

Embodiment 3

[0015] A method for preparing an organic selenium enzyme preparation with a high conversion rate, the method is as follows: 83 parts by weight of bran, 12 parts by weight of soybean meal, 1.0 part by weight of rice husk powder, 0.5 parts by weight of ammonium sulfate, and 0.5 parts by weight of potassium dihydrogen phosphate 3 parts by weight of malt, 3 parts by weight of malt, the proportioning of 100 parts by weight of sodium selenite solution with a concentration of 0.15% with the above-mentioned materials to obtain a mixture, after stirring evenly, when the water content of the mixture is not enough, use sterile water to Add water to the mixture so that its water content is 60% by weight, then, under the condition of 0.20MPa and 120°C, carry out high temperature and high pressure sterilization for 45 minutes, after the sterilization, cool the above materials to At 37°C, inoculate 2.5% of its weight of Candida utilis (Candidautilis) aspergillus strains, stir evenly and culti...

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Abstract

The invention provides a preparation method of an organic selenium enzyme preparation with high conversion rate. The preparation method of the organic selenium enzyme preparation with high conversionrate comprises the following steps of mixing bran, soybean meal, rice husk powder, a sodium selenite solution and the like; carrying out high-temperature high-pressure sterilization; inoculating a koji-plate bacterial strain of torula yeast into the sterilized mixture, carrying out culturing, and taking out the culture product; inoculating a koji-plate bacterial strain of aspergillus usamii onto the culture product, and carrying out second culturing; inoculating a koji-plate bacterial strain of bacillus subtilis onto the culture product, and carrying out third culturing; and then, drying the culture product of the third culturing, and carrying out crushing so as to obtain the organic selenium enzyme preparation. According to the preparation method of the organic selenium enzyme preparationwith high conversion rate, three times of conversion are continuously carried out by adopting three bacterial strains so that the conversion rate of inorganic selenium into organic selenium is high,namely 98% or above; and thus, the inorganic selenium with extremely low potency is conversed into organic selenium which has relatively high potency. The prepared organic selenium enzyme preparationintegrates organic trace elements, enzyme preparations and probiotics; and thus, the preparation has a plurality of biological functions, as well as the functions of reducing anti-nutritional factors.

Description

technical field [0001] The invention provides a preparation method of an organic selenium enzyme preparation with high conversion rate. Background technique [0002] At present, most of the selenium products sold in the feed additive market are inorganic selenium, that is, sodium selenite, which has low potency when used, and long-term use will pollute water quality and the environment. Although there is a part of organic selenium, most of them are industrially synthesized products with high cost and expensive price. Now there is a preparation method of an organic selenium enzyme preparation, which uses probiotics such as fungi and bacteria as carriers to convert inorganic selenium into organic selenium through biotransformation, thereby improving the potency and avoiding the pollution caused by the use of inorganic selenium. However, this preparation method usually only uses one kind of bacteria for one conversion, and the conversion rate of inorganic selenium into organic...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/189A23K20/20
CPCA23K20/189A23K10/18A23K20/30
Inventor 不公告发明人
Owner 郑州助协生物科技有限公司
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