Method for improving RA content of stevia rebaudiana through in-vitro culture

A technology of in vitro culture and culture method, applied in the field of cell biology and biology, to achieve the effect of sweet taste and increasing RA content

Inactive Publication Date: 2018-06-05
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the study of increasing the RA content of Stevia rebaudiana leaves through callus

Method used

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  • Method for improving RA content of stevia rebaudiana through in-vitro culture
  • Method for improving RA content of stevia rebaudiana through in-vitro culture
  • Method for improving RA content of stevia rebaudiana through in-vitro culture

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Embodiment Construction

[0021] In order to make the means, creation features, objectives and effects of the present invention easy to understand, the present invention will be further elaborated below.

[0022] After collecting excellent stevia varieties in China and detecting the RA content by liquid chromatography, it was determined to use the variety Mingguang No. 1 stevia variety with high RA content. The leaves were collected and sterilized according to the conventional tissue culture disinfection procedures.

[0023] Add 200 mg / g RA solution to the callus medium.

[0024] Then inoculate.

[0025] The ambient temperature of the tissue culture room was maintained at 25°C, the relative humidity was 75%, and the day was 12h / night 12h.

[0026] After about 30 days to produce green callus, transfer to shoot induction medium for culture.

[0027] After about 30 days to produce young shoots, they were transferred to rooting medium for cultivation.

[0028] Then it is transplanted into the flower pot...

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Abstract

The invention discloses a method for improving RA content of stevia rebaudiana through in-vitro culture. The method comprises the following steps: inoculating stevia rebaudiana leaves onto a callus induced medium, transferring the produced callus into a differential medium, forming buds, transferring into a rooting medium, growing a root system, acclimatizating and transplanting into a greenhouse,and performing routine cultivation management. The invention belongs to the technical fields of cytobiology and biology. The invention provides a method for rapidly obtaining stevia rebaudiana plantswith high RA content, the content of the RA in the stevia rebaudiana leaves is increased, and the taste is excellent.

Description

technical field [0001] The invention relates to a method for in vitro culture of Stevia rebaudiana Bertoni to increase RA content in leaves of Stevia rebaudiana, belonging to the field of cell biology and biology. Background technique [0002] Stevia rebaudiana Bertoni (Stevia rebaudiana Bertoni) is a perennial herb of the genus Stevia in the Asteraceae family. Stevia is native to the Paraguay region of South America, and its leaves contain stevioside (stevioside, ST), which is 200-300 times sweeter than sucrose, and is a natural sweet substance with high sweetness and low energy. It has been proved by modern science that stevia has a good preventive effect on patients with high blood pressure, diabetes, arteriosclerosis and coronary heart disease. Drinking stevia tea can help relieve mental fatigue, promote metabolism, and benefit human health. It is a modern safe and healthy sugar . At present, studies have shown that steviol glycosides include rebaudioside A (Rebaudiosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郑成淑唐琪王珍珍
Owner SHANDONG AGRICULTURAL UNIVERSITY
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