KRAS gene detection kit and detection method

Abstract

The invention relates to the fields of gene engineering and molecule biology, and provides a KRAS gene detection kit and a detection method. The kit has the advantages that a specific amplification primer sequence is designed according to the specific sites of No.2 exon and No.3 exon regions of KRAS, so that the false positive rate of the to-be-detected sites is low in detection, the sensitivity is high, and the gene mutation with mutation ratio of 5% or above can be detected; library molecules are respectively obtained from an F terminal and a R terminal at both ends of a KRAS nucleic acid segment of the to-be-detected site, and the F terminal and the R terminal respectively contain amplification primer binding sites; when the KRAS nucleic acid segment containing the multiple to-be-detected sites is sequenced, the amplification primers for amplifying the library molecules are unified, the consistency in amplification efficiency is guaranteed, and the difficulty in design of the primeris decreased; the R terminal is of a hairclip structure, and can be used for sealing the tail end of the corresponding amplification product, so that the F terminal is effectively prevented from being connected with the R terminal, and the connecting efficiency is improved.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, and more specifically relates to a KRAS gene detection kit and detection method. Background technique [0002] KRAS is an important proto-oncogene, which acts as a "switch" in the signal transduction pathway of tumor cell growth, proliferation and angiogenesis, affecting the growth and spread of tumors. KRAS gene detection helps to select colorectal cancer patients who are effective against anti-EGFR (epidermal growth factor receptor) targeted therapy drugs, thus helping doctors choose the most effective treatment for tumor patients. [0003] The KRAS gene is divided into two types: normal state (called wild type) and abnormal state (mutant type). The normal KRAS gene can inhibit the growth of tumor cells. Once a mutation occurs, it will continue to stimulate cell growth, disrupt the growth pattern, and lead to tumor formation. By detecting the patient's KRAS gene, the ...

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Problems solved by technology

[0006] The object of the present invention is to provide a KRAS gene detection kit and detection method, aiming to solve the problems of low sensitivity and low accuracy of KRAS detection in the prior art

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