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Harvesting method of selenium-enriched chlorella product via diatomite-based positively charged green flocculant

A harvesting method, chlorella technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve problems such as adverse effects on human health, achieve compact flocs, convenient operation, and a preparation method simple effect

Active Publication Date: 2018-05-18
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Commonly used chemical flocculants are mainly inorganic polymers (polyferric chloride, polyaluminum chloride, etc.), but the addition of metal cations will cause adverse effects on human health

Method used

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  • Harvesting method of selenium-enriched chlorella product via diatomite-based positively charged green flocculant
  • Harvesting method of selenium-enriched chlorella product via diatomite-based positively charged green flocculant
  • Harvesting method of selenium-enriched chlorella product via diatomite-based positively charged green flocculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Cultivation of Chlorella pyrenoidosa with a medium containing selenite:

[0038] Chlorella pyrenoidosa (algae species were purchased from Wild Biological Germplasm-Freshwater Algae Acquisition Bank of Chinese Academy of Sciences) was selected. Inoculate Chlorella pyrenoidosa into BG11 culture medium (including NaNO 3 1.5g / L, KH 2 PO 4 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 ·H 2 O 0.036g / L, citric acid 0.006 g / L, ferric ammonium citrate 0.006g / L, EDTANa 2 0.001g / L, NaCO 3 0.02g / L, A5 1ml / L), the inoculum size is 10%, and the initial density is 6×10 6 Individual / mL, one-time addition of sodium selenite in the culture solution, its concentration is respectively 0mg / L, 5mg / L, 8mg / L, 10mg / L, 20mg / L. Cultivate at a temperature of 20-25°C, pH=7.2, light intensity 2000-3000lux, and 24 hours of light per day. The culture time is 7 days. The algae liquid is taken regularly every day to measure the absorbance at 680nm and observe different concentrations of selenous ...

Embodiment 2

[0040] A method for harvesting selenium-enriched chlorella products with a diatomite-based positively charged green flocculant, the specific steps are:

[0041] (1) Preparation of diatomite-based positively charged green flocculant:

[0042] Chitosan 1.5g is dissolved in the hydrochloric acid solution of 5mol / L 50ml and stirs until completely dissolving, obtains chitosan-hydrochloric acid solution, adds diatomite in chitosan-hydrochloric acid solution, the added diatomite and shell The mass ratio of polysaccharides is 1:6. Stir at room temperature for 24 hours and then filter, then dry at 75°C, grind, and pass through a 200-mesh sieve to obtain a diatomite-based positively charged green flocculant.

[0043] (2) Cultivate Chlorella pyrenoidosa with a culture solution containing selenite: Chlorella pyrenoidosa (algae species purchased from Wild Biological Germplasm-Freshwater Algae Species Acquisition Bank, Chinese Academy of Sciences) was used. Inoculate Chlorella pyrenoidosa ...

Embodiment 3

[0048] A method for harvesting selenium-enriched chlorella products with a diatomite-based positively charged green flocculant, the specific steps are:

[0049] Chlorella pyrenoidosa (algae species purchased from Wild Biology Germplasm-Freshwater Algae Acquisition Bank, Chinese Academy of Sciences) was used. Inoculate Chlorella pyrenoidosa into BG11 culture medium (including NaNO 3 1.5g / L, KH 2 PO 4 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 ·H 2 O 0.036g / L, citric acid 0.006g / L, ferric ammonium citrate 0.006g / L, EDTANa 2 0.001g / L, NaCO 3 0.02g / L, A5 1ml / L), the inoculum size is 10%, and the initial density is 6×10 6 Individual / mL, sodium selenite is added once in the described culture solution, and its concentration is 5mg / L. The culture is carried out under the conditions of temperature 20-25°C, pH=7.2, light intensity 2000-3000 lux, and 24 hours of light every day. The culture time is 5 days, and the chlorella liquid after selenium-enriched culture is collected. ...

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Abstract

The invention provides a harvesting method of a selenium-enriched chlorella product via a diatomite-based positively charged green flocculant. The method is characterized by comprising the following steps: cultivating chlorella pyrenoidosa in a culture solution containing selenite, so that chlorella liquid undergoing selenium-enriched culture is obtained; and adding the diatomite-based positivelycharged green flocculant to the chlorella liquid undergoing selenium-enriched culture, standing by, discarding supernatant liquid and harvesting the selenium-enriched chlorella. According to the method, by harvesting and cultivating the selenium-enriched chlorella, the finished product that an organic selenium content reaches 73% or above can be obtained, and the method is simple in culture steps,low in cost and convenient to operate. In addition, the selenium-enriched chlorella product that the algae flocculant which is free from toxins and harm is adopted is simple in preparation method, green-going and free from pollution, and a chlorella harvesting rate can reach 90-97%. The method is applicable to large-scale production; and moreover, the obtained product is applicable to the fieldsof food health care and medicine, and a certain economic value is created.

Description

technical field [0001] The invention belongs to a method for preparing organic selenium-rich products that can be applied in the fields of food health care and medical treatment. Green flocculants can also be developed accordingly for the flocculation and removal of blue-green algae when nutrient-rich waters explode. Specifically, it is The invention relates to a method for cultivating selenium-enriched chlorella and a method for preparing a green and pollution-free microalgae flocculant. Background technique [0002] Selenium is a natural trace element and one of the essential elements for the human body and other mammals. Selenium has physiological functions such as protecting biofilm, scavenging free radicals, anti-cancer, anti-aging, and enhancing immunity. However, inorganic selenium is toxic, and its dosage and range are strictly controlled. Excessive intake of inorganic selenium may lead to poisoning, while lack of selenium can lead to Keshan disease, Kaschin-Beck dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 宋新山赵雨枫朱梦婷王宇晖王培娟谷琪赵志淼王俊峰谢璟张璐璐
Owner DONGHUA UNIV
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