A method of uplc-ms-ms for rapid screening of the difference between different genetic sources of Artemisia caprinarius and Artemisia bismuth

Abstract

The invention discloses a method for rapid variation screening of different base sources of artemisia capillaris and artemisia scoparia through UPLC-MS-MS. The method comprises the following steps: 1)collecting a to-be-distinguished artemisia capillaris and artemisia scoparia medicinal materials, preparing a standard decoction sample; 2) introducing the sample in a UPLC-MS-MS system for analysisto obtain the corresponding mass spectrum data; 3) directly introducing the primary collected data in Compound Discoverer 2.1, and performing data analysis; 4) employing a dynamic program calculationmethod to correct chromatographic peak drift, aligning chromatographic peaks, using a proximal clustering method for registering a substance with approached retention time to a compound; and 5) usinga variance analysis to calculate the variation substance, and determining the variation of different base sources. The compound mass spectrum information is output to an original file, transition to other format cannot be required, the compound mass spectrum information can be directly introduced to Compound Discoverer 2.2 database to search the compound, the method can rapidly lock the variationcompound in the traditional Chinese medicinal material samples, and is suitable for rapid analysis and determination of the large-batch samples.

Description

technical field [0001] The invention relates to the technical field of research on components of traditional Chinese medicines, in particular to a UPLC-MS-MS method for rapidly screening the difference of different base sources of Artemisia caprifolia and Artemisia basilicum. Background technique [0002] Capillaris is the dry aboveground part of Artemisia scoparia Waldst.etKit. or Artemisia capillaris Thunb., a perennial plant of the Compositae family. According to different harvest seasons, there are two types of capillaris recorded in the 2015 edition of the Chinese Pharmacopoeia, which are cotton Yinchen and flower Yinchen. The capillary capillary harvested in spring is called mian capillary, and the capillary capillary harvested in autumn is called flower capsicum. [0003] Yinchen is a commonly used traditional Chinese medicine, first recorded in "Shen Nong's Materia Medica", listed as top grade, has the effects of clearing away heat and promoting dampness, promoting ...

AI Technical Summary

Problems solved by technology

However, there are a large number of false positives and false negatives in these methods, which lead to the omission of important substance information when using these methods for analysis, which in turn leads to the inability of the screened differential substances to effectively characterize group differences. Difficulties in identification of the same kind of Chinese herbal medicines

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