Experimental method for spectrally detecting soluble organic components of plant extract

Abstract

The invention relates to an experimental method for spectrally detecting soluble organic components of plant extract. The experimental method comprises the following steps: separately extracting organic acids, sugar substances, macromolecular organic matters and micromolecular alcohols, then performing liquid-phase detection or gas-phase detection, wherein the extracting step specifically comprises the following steps: preparing raw materials, sealing extracting, ultrasonically treating extract liquid, centrifuging the extract liquid, and extracting an organic phase. Compared with the prior art, the type identification and quantitative analysis of various soluble organic components of the plant extract liquid can be realized, the influence of the variation of an independent variable on a response value can be intuitively known, the interaction of the independent variables can be described, a reliable conclusion can be provided for a multi-factor experiment of the mixed substance detection and interaction, the recovery rate, the repetition, and the detection amount and the detection types of the organic matters can be increased; and an optimum separation effect can be achieved for the substances in the extract liquid, a spectrum detection peak value is clear, no tailing phenomenon exists, and the overlapping influence of a complexing agent in the traditional method for micromolecular substances can be avoided.

Description

technical field [0001] The invention relates to an experimental test method for detecting organic matter by using a chromatographic instrument, in particular to an experimental method for chromatographic detection of soluble organic components in plant extracts. Background technique [0002] At present, although the research on improving the detection efficiency of chromatographic organic components by changing the extraction conditions has been reported, the reports are mostly on the detection of single-type samples with clear targets, and this method is not suitable for mixed substances and complex samples with unclear targets. The detection may have unreliable conclusions. In addition, the specific and selective micro-extraction device used for extraction is easy to operate and has high detection accuracy. However, when using this device to extract matrix samples in complex environments, the target pollutants are easily mixed with other substances in the sample matrix. S...

AI Technical Summary

Problems solved by technology

[0004] The above invention has many beneficial effects, but there is a complex carbon rod production process, which needs to be realized under specific chemical environmental conditions, which increases its production steps and cost; in addition, although it is suitable for extraction in complex environments, it is only suitable for extraction targets The extraction and detection of organic matter under clear conditions; second, the above-mentioned patents are highly targeted, and it is difficult to detect various organic matter components in complex leachate

Images