A detection kit for 1,5-sorbitol and its detection method

A technology of sorbitan and detection kits, applied in biochemical equipment and methods, microbiological determination/inspection, measuring devices, etc., can solve the problems of inaccurate detection of low-concentration samples, insufficient glucose energy supply, weak detection signals, etc. , to achieve the effects of strong reagent detection signal, improved specificity and good accuracy

Active Publication Date: 2019-01-04
GUANGZHOU JINDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a detection kit for 1,5-sorbitol, which solves the problem of insufficient energy supply in the glucose elimination process in the kit, by-product glucose 6-phosphate, Pyruvate accumulation causes negative interference, weak detection signal and inaccurate detection of low concentration samples

Method used

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  • A detection kit for 1,5-sorbitol and its detection method
  • A detection kit for 1,5-sorbitol and its detection method
  • A detection kit for 1,5-sorbitol and its detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The 1,5-sorbitan detection kit of this embodiment is:

[0062] Reagent R1 includes the following components at the following concentrations:

[0063]

[0064]

[0065] Reagent R2 includes the following components in concentrations:

[0066] Phosphate buffer (pH 7.4) 10mmol / L

[0067] Pyranose Oxidase 6KU / L

[0068] Reagent R3:

[0069] Luminescent substrate: 1,2-dioxetane boronic acid.

[0070] The detection method of the detection kit of this embodiment is:

[0071] Add (1) deionized water, (2) test solution containing 300μmol / L 1,5-AG, (3) test solution containing 40mmol / L glucose, and (4) test solution containing 300μmol / L glucose into 4 reaction cups. / L 1,5-AG and 40mmol / L glucose test solution 5μL each, then inject 50μL reagent R1 respectively, after heating at 37℃ for 3 minutes, add 100μL reagent R2 to each reaction cup, and continue heating at 37℃ for 3 minutes , Add 10 μL reagent R3 luminescent substrate to each reaction cup, and continue heating at 37°C for 3 minutes, th...

Embodiment 2

[0076] As an embodiment of the 1,5-sorbitan detection kit of the present invention, the components and dosage of the 1,5-sorbitan detection kit of this embodiment are:

[0077] Configure 1,5-AG diagnostic kit according to the following ingredients and dosage.

[0078] The kit according to Example 1 is added to the pyruvate removal system and configured as a 1,5-AG detection kit.

[0079] Reagent R1 includes the following components at the following concentrations:

[0080]

[0081] Reagent R2 includes the following components in concentrations:

[0082] Phosphate buffer (pH 7.4) 10mmol / L

[0083] Pyranose Oxidase 6KU / L

[0084] Reagent R3:

[0085] Luminescent substrate: 1,2-dioxetane boronic acid.

[0086] Use the detection method of Example 1 to detect the sugar removal system.

[0087] Table 2

[0088]

[0089] It can be seen from Table 2 that the light signal intensity of (1) and (3) are almost the same, indicating that the kit of the present invention can completely eliminate 40mmol / L...

Embodiment 3

[0091] As an embodiment of the detection kit for 1,5-sorbitol of the present invention, the detection kit for 1,5-sorbitol of this embodiment is:

[0092] Reagent R1 includes the following components at the following concentrations:

[0093]

[0094] Reagent R2 includes the following components in concentrations:

[0095] Tris-HCl buffer (pH 7.4) 100mmol / L

[0096] Pyranose Oxidase 20KU / L

[0097] Sodium azide 0.1wt%

[0098] Reagent R3:

[0099] Luminescent substrate: 1,2-dioxetane boronic acid.

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Abstract

A test kit for 1,5-anhydro-D-glucitol (1,5-AG) and a detection method thereof. The test kit can implement an energy supply of a sample in a reaction cycle process, eliminate the interference of glucose and test byproducts of glucose 6-phosphate and pyruvic acid in the sample, and avoid a reverse reaction caused by the product accumulation. After the glucose and the byproduct accumulation are eliminated, 1,5-AG is converted into 1,5-anhydro-fructose and hydrogen peroxide. Hydrogen peroxide reacts with a chemiluminescent substrate to form a continuously stable chemiluminescence signal. Compared with the traditional enzymatic colorimetric method, the test kit has advantages of high sensitivity, high accuracy, high specificity, strong anti-interference, good stability, etc.

Description

Technical field [0001] The invention relates to the technical field of biochemical reagents, in particular to a 1,5-sorbitan detection kit and a detection method thereof. Background technique [0002] 1,5-AG (1,5-sorbitan) has a significant negative correlation with existing diabetes indicators, and the reduction of 1,5-AG in the blood is significantly related to the severity of diabetes. The most significant difference between 1,5-AG and other indicators is the different cycle that reflects the change of diabetes. Blood glucose (GLU) reflects the patient's blood glucose status at the time, glycosylated albumin (GA) reflects the patient's average blood glucose status within half a month, and glycosylated hemoglobin (HbAlc) reflects the patient's average blood glucose level in the past 2 to 3 months, while 1,5-AG can reflect the average blood glucose level in the past few days to 1 week. [0003] At present, the domestic and foreign markets mainly adopt the enzymatic method to dete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/48C12Q1/32
CPCC12Q1/32C12Q1/48G01N2333/902G01N2333/91215
Inventor 张玲李民友梁灿蔺涛
Owner GUANGZHOU JINDE BIOTECH
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