Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker influencing growth of pig main trotter and application of molecular marker

A technology of molecular markers and main hooves, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems that no one has discovered the main hooves of pigs, and achieve early identification, which is convenient and easy to increase. Breeding progress, effects of important economic benefits

Active Publication Date: 2018-05-08
SOUTH CHINA AGRI UNIV +1
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no one has found any molecular markers for pig main hoof traits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker influencing growth of pig main trotter and application of molecular marker
  • Molecular marker influencing growth of pig main trotter and application of molecular marker
  • Molecular marker influencing growth of pig main trotter and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 is to explain in detail the inventive process of obtaining the pig's main hoof growth situation in the present invention.

[0029] Phenotype data collection: Accurately measure the length of the pig’s main hoof with a tape measure, accurate to millimeters, keep the standard consistent during the measurement process, and make a record of the length. Because the length of the main hoof is greater than 10cm, it is very easy to cause damage to the limbs and hooves and be eliminated. Therefore, we define the length of the main hoof as 10cm as the overgrowth of the main hoof. The above experiment was carried out in the East China Breeding Breeding Farm of Guangdong Wenshi Food Group Co., Ltd. All Large White sows were housed in stalls with dimensions of 2.1m×0.7m×1.1m in length×width×height, and had free access to drinking water. , and according to the unified feeding standard, unified diet feeding, regular and quantitative feed intake.

Embodiment 2

[0030] Example 2 is to specifically explain the inventive process of obtaining the gene marker in the present invention.

[0031] (1) Tissue DNA extraction and quality control: the ear tissue of the Large White sow in the above-mentioned Example 1 was collected, soaked in 75% ethanol in time and placed in a -20°C refrigerator for later use. The whole genome DNA of the large white sow was extracted according to the phenol-chloroform method, and the concentration and quality of the DNA of the large white sow were determined by Nanodrop-ND1000 nucleic acid concentration instrument and agarose gel electrophoresis. Specifically, the A260 / 280 ratio measured by the nucleic acid concentration meter is 1.8-2.0, and the A260 / 230 ratio is 1.7-1.9. It is judged that the purity is qualified, and the concentration is higher than 300 ng / microliter. It is judged that the concentration is qualified; the purity and DNA samples with qualified concentrations were uniformly diluted to 50 ng / μl. T...

Embodiment 3

[0034] Embodiment 3 specifically explains the inventive process of the invention for detecting SNP markers.

[0035] (1) Amplification of the target fragment containing the SNP site significantly related to the growth of the main hoof of Large White pig The target fragment is a 310bp nucleotide sequence in chromosome 16, and the upstream and downstream primers for sequence amplification are:

[0036] SEQ ID NO:2 upstream primer 5'-TCTCTGCTGTGGTGAATATC-3'

[0037] SEQ ID NO:3 downstream primer 5'-ACTCTGTCTTGGTGTGTTC-3'

[0038] (2) PCR amplification system and condition setting: Configure 10ul system, including 1ul DNA sample, 0.2ul upstream primer, 0.2ul downstream primer, PCR Mix 5ul, ddH 2 O 3.6ul; PCR conditions were set as pre-denaturation at 95°C for 3min, denaturation at 95°C for 30s, annealing at 55°C for 30s, extension at 72°C for 60s, a total of 36 cycles, and a final extension at 72°C for 7min.

[0039] (3) DNA sequence sequencing detection: PCR products were sent to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of domestic animal molecular marker preparation, in particular to an SNP marker influencing the growth of a pig main trotter and application of the SNP marker. The SNP marker is locus mutation of the 52293663 th basic group of the 16 th chromosome of a pig genome from A to G and corresponds to the 218bp th in the sequence shown in SEQ ID NO: 1. The molecular genetic marker can be used for screening the growth conditions of main trotters of boars, can effectively reduce the probability that the boars are eliminated after leg and foot diseases are caused when the main trotters overgrow in actual production, effectively prolong the life of the boars and increase the economic benefit.

Description

technical field [0001] The invention belongs to the field of molecular marker-assisted selection technology and animal genetic breeding, and particularly relates to a molecular marker that affects the growth traits of pig main hooves and its application. Background technique [0002] Pigs (Susscrofa) are mammals of the order Artiodactyla. The 3rd and 4th toes are particularly developed and equal in length, called the main hoof ( figure 1 ). The main hoof is mainly used to bear the weight of the pig, and plays an important supporting role in the process of walking and standing of the pig. Usually, the main hoof length of sows is about 5 cm, while the length of overgrown main hoof can reach 13 cm. The disadvantage of too long main hoof is that it is easy to cause mechanical damage, and because of the special structure of the hoof, it is difficult to recover from the damage, which in turn will affect the standing and eating of pigs, and increase the probability of abortion in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨杰吴珍芳全建平郑恩琴蔡更元杨明
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com