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Multi-stroma Cordyceps militaris culture medium and use method thereof

A culture medium, Cordyceps fungus technology, applied in microorganism-based methods, biochemical equipment and methods, fungi and other directions, can solve the problems of decreased viability, slow color change, slow mycelium growth, etc., to solve the problem of slow growth , reducing the effect of mutual competition inhibition, shortening the effect of culture time

Inactive Publication Date: 2018-05-08
龙里县康雅先蔬菜种植农场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, the artificial cultivation and development of Cordyceps fungus has developed rapidly. However, after several generations of cultivation, the strains of Cordyceps fungus will show slow mycelium growth, slow color change, and decline in vitality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: a kind of multiseat Cordyceps culture medium, comprises sweet potato puree 87g / L, peptone 18g / L, glucose 6g / L, potassium dihydrogen phosphate 1.5g / L, sodium chloride 0.7g / L, magnesium sulfate 0.95 g / L, agar 3g / L.

Embodiment 2

[0017] Embodiment 2: a kind of multiseat Cordyceps culture medium, comprises sweet potato puree 80g / L, peptone 20g / L, glucose 5g / L, potassium dihydrogen phosphate 1.8g / L, sodium chloride 0.6g / L, magnesium sulfate 1.2g / L, agar 2.5g / L.

Embodiment 3

[0018] Embodiment 3: a kind of multiseat Cordyceps culture medium, comprises sweet potato puree 95g / L, peptone 15g / L, glucose 8g / L, potassium dihydrogen phosphate 1.2g / L, sodium chloride 1g / L, magnesium sulfate 0.8 g / L, agar 3.8g / L.

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PUM

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Abstract

The invention discloses a multi-stroma Cordyceps militaris culture medium and a use method thereof, wherein the culture medium comprises 80-95 g / L of sweet potato mud, 15-20 g / L of peptone, 5-8 g / L ofglucose, 1.2-1.8 g / L of potassium dihydrogen phosphate, 0.6-1 g / L of sodium chloride, 0.8-1.2 g / L of magnesium sulfate, and 2.5-3.8 g / L of agar. According to the present invention, the culture mediumis reasonably prepared, and the single strain is used, such that the competitive inhibition among multiple strains during the culture can be reduced, the culture times of the strain and the sporophyte are greatly shortened, and the problems of slow growth, slow color transformation and declining vitality of mycelium are solved.

Description

technical field [0001] The invention relates to a culture medium and a method for using it, in particular to a culture medium for Cordyceps multiseaus and a method for using it. Background technique [0002] Cordyceps fungus is a type of fungus that lives in the body of insects. It infects insects through spore transmission, contact infection, etc., and uses the nutrients in the insect's body to mature quickly, causing the insect to die. [0003] In recent years, the artificial cultivation and development of Cordyceps fungus has developed rapidly. However, after several generations of cultivation, the strains of Cordyceps fungus will show slow mycelium growth, slow color change, and decline in vitality. Contents of the invention [0004] The object of the present invention is to provide a multi-seat Cordyceps culture medium and its use method in order to solve the above technical problems. [0005] Technical scheme of the present invention: a kind of multiseat Cordyceps c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 苟荣祥
Owner 龙里县康雅先蔬菜种植农场
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