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Method for increasing content of tanshinone in salviae miltiorrhizae roots through salt stress

A technology for tanshinone content and salvia is applied in the field of increasing tanshinone content in salvia roots, and achieves the effects of simple operation, increasing tanshinone content and simple method.

Inactive Publication Date: 2018-05-08
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the effect of salt stress on the dynamic accumulation of tanshinone

Method used

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  • Method for increasing content of tanshinone in salviae miltiorrhizae roots through salt stress

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Experimental program
Comparison scheme
Effect test

Embodiment 2

[0019] NaCl Stress Treatment of Salvia Miltiorrhiza

[0020] Robust Salvia miltiorrhiza plants growing in a liquid culture environment are selected and treated with salt stress so that the final concentration is 50-100 mM; meanwhile, Salvia miltiorrhiza treated with water is used as a control. The roots of Salvia miltiorrhiza were collected 8 hours after treatment, and the content of tanshinone was determined by high performance liquid chromatography, and each treatment was repeated 5 times. The liquid culture refers to the addition of 30 g / L sucrose to 1 / 2 MS culture liquid.

Embodiment 3

[0022] Utilize HPLC to measure the content of tanshinone in the root of salvia miltiorrhiza:

[0023] (1) Liquid chromatography conditions:

[0024] The content of tanshinone was determined by high-performance liquid chromatography, 5 µm Eclipse Plus C18 silica gel reversed-phase column, 4.6×150 mm, the mobile phase was methanol:water (75:25), the flow rate was 1mL / min, and the detection wavelength was 280 nm. The ingredients are Tanshinone I, Tanshinone IIA, and Cryptotanshinone.

[0025] (2) Preparation of standard curve

[0026] Tanshinone I, Tanshinone IIA, and cryptotanshinone standard products were all purchased from the National Institute of Pharmaceutical and Biological Products. Standard samples with concentrations of 1, 10, 20, 50, and 100 µg / mL were prepared with chromatographic methanol, and the peak time and peak area were recorded after injection. The retention times of tanshinone I, tanshinone IIA, and cryptotanshinone are 7.8, 13.8, and 8.5 min, respectively...

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Abstract

The invention provides a method for increasing the content of tanshinone. The method specifically comprises the following steps of selecting salviae miltiorrhizae plants robust in growth, and conducting liquid culture for two months to make the salviae miltiorrhizae plants adapt to the aquatic environment; then adding NaCl into a culture solution so that after stress treatment is conducted for 8 hours, the content of tanshinone in salviae miltiorrhizae roots can be increased. By means of the method for increasing the content of tanshinone in the salviae miltiorrhizae roots through NaCl stresstreatment, a liquid culture method is adopted, NaCl is added into the culture solution, and the salviae miltiorrhizae plants of which the content of tanshinone is increased are obtained. After the NaCl stress treatment, the content of detected total tanshinone (tanshinone I, tanshinone IIA and cryptotanshinone) detected in the salviae miltiorrhizae roots is obviously increased compared with a control group. The average content of tanshinone in the salviae miltiorrhizae roots subjected to the NaCl stress treatment is 1.3 times or above of that in the control group. The content of tanshinone insalviae miltiorrhizae roots can be effectively increased, and the method is easy to operate and low in cost and provides technical reference for saline-alkali soil cultivation of a medicinal plant ofsalviae miltiorrhizae.

Description

technical field [0001] The invention belongs to the technical field of salvia miltiorrhiza cultivation, and in particular relates to a method for increasing the content of tanshinone in roots of salvia miltiorrhiza. Background technique [0002] Salvia ( Salvia miltiorrhiza Bge .) for Lamiaceae ( Labiatae ) Salvia ( Salvia ) is a perennial herbal medicinal plant whose roots and rhizomes are used as medicine. It is a commonly used bulk Chinese herbal medicine in my country. Salvia mainly contains two types of active ingredients: one is water-soluble phenolic acids; the other is fat-soluble diterpene quinones, namely tanshinones. Modern medical research shows that tanshinone has a significant effect in the treatment of cardiovascular and cerebrovascular diseases. [0003] Salt is one of the important environmental factors that affect plant growth and development. In adversity, it can reduce the osmotic potential of the environment and cause plant cells to lose water, res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 尉万聪赵宪争岳东霞杨迎霞
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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