Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A rapid detection method and kit for dicofol residues

A technology of dicofol and a detection kit, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of long operation time, weak reactivity, and low accuracy. , to achieve accurate experimental results, high sensitivity, and improve the effect of color rendering

Active Publication Date: 2020-03-31
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing rapid detection methods for dicofol use the pyridine-alkaline colorimetric method, and pyridine is a volatile and odorous toxic solvent, which will cause certain harm to the human body
At the same time, the accuracy of this method is not high, the operation time is long, and its application is limited
[0004] The reactivity of bipyridine is far weaker than that of pyridine, and it basically does not react with chloroform to develop color. There is no use of bipyridine to detect dicofol in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A rapid detection method and kit for dicofol residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] Steps

[0044] 1) Extraction-Extraction: Weigh 2.5g of sample into a centrifuge tube, add 10mL of n-hexane, shake up and down for 30s, draw the extract through the solid-phase extraction column, dry it, and elute the solid-phase column with 1mL of acetonitrile;

[0045] 2) Color development by reaction: Add 0.3g of 2,2-bipyridine, 30uL of sodium hydroxide solution and 50μL of tetramethylammonium hydroxide solution, heat in a water bath at 80-100°C for 2min. A yellow color indicates the presence of dicofol.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a dicofol residue rapid detection method and a kit thereof. The dicofol residue rapid detection method comprises the following steps: extracting a sample, extracting a solid phase, after extracting a sample extract through a solid-phase extraction column, eluting the sample extract by an eluate, adding 2,2-dipyridine, a strong alkaline solution and tetramethylammonium hydroxide in the eluted eluate, and performing a chromogenic reaction and colorimetric determination to obtain the dicofol amount. According to the invention, sample detection can be completed by only 20 min, dipyridine having the characteristics of odorlessness and safety is taken as a reaction developer, and an experiment method realizes green environmental protection; tetramethylammonium hydroxide is added as a reaction catalyst, so that the chromogenic reaction can be completed in two minutes, and the chromogenic effect is enhanced; the method determines the dicofol content by comparing the colors, sensitivity is high, and the method can be widely used for rapidly detecting dicofol in samples such as vegetables, fruits, and tea.

Description

technical field [0001] The invention relates to a detection method and kit for pesticide residues, in particular to a color reaction-based rapid detection method for dicofol and a kit. Background technique [0002] Dicofol is a broad-spectrum and low-toxicity organochlorine acaricide, which is mainly used to prevent and control a variety of acarids in cotton, fruit trees, flowers, etc. Since dicofol is not easy to decompose and has a high residue, it is not suitable for use in tea, vegetables and edible fungi. However, the use of dicofol in my country is still relatively common at present, and the detection rate of dicofol in tea, vegetables and edible fungi is relatively high. [0003] Dicofol will undergo alkaline hydrolysis under alkaline conditions to produce chloroform and other substances. Chloroform will react in the pyridine-NaOH solution system to form a red compound, and the concentration of dicofol can be calculated according to the absorbance value of the red c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 吴沛堂谢俊平梁科卢新石松温林凤
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com