Triple real-time fluorescent PCR primer and probe used for identifying high pathogenicity H7N9 influenza virus

Abstract

The invention provides a set of triple real-time fluorescent PCR primer and probe used for identifying high pathogenicity H7N9 influenza virus. The set of primer and probe comprises a universal influenza virus FQ-PCR primer and a probe (Seq ID No.1-3), an influenza virus HA gene FQ-PCR primer and a probe (Seq ID No.4-6) and an influenza virus NA gene FQ-PCR primer and a probe (Seq ID No.7-9). Three pairs of PCR primers can carry out amplification in the same PCR reaction tube at the same time, triple detection is realized, optimization is carried out, and then rapid molecular identification onhigh pathogenicity H7N9 influenza virus HA and NA genes can be completed within 2 hours, so that the set of primer and probe have the advantages of speediness, simplicity, specificity, high sensitivity, low cost and stable result and is significant to the aspects of early warning on epidemic situation of an H7N9 high pathogenicity influenza virus variant strain separated in 2017 and pathogen monitoring of an epidemic area.

Description

technical field [0001] The invention relates to the detection of H7N9 subtype influenza virus, in particular to a set of triple real-time fluorescent PCR primers and probes for identifying highly pathogenic H7N9 influenza virus triple real-time fluorescent PCR primers for identifying highly pathogenic H7N9 influenza virus and probes. Background technique [0002] Avian Influenza (AI) is an acute and severe infectious disease of poultry caused by Influenza Virus Type A. In 1878, Porron Cito was first reported in Italy. In 1955, Schafer first proved that the pathogen was influenza A virus. At first, the disease was popular in many countries in Europe, and later occurred in some countries in the Americas, Africa, and Asia. In recent years, different subtypes of avian influenza viruses have been isolated in some areas of my country. Normally, avian influenza virus does not infect humans, but since the avian influenza virus H5N1 infected humans in 1997, there have been report...

AI Technical Summary

Problems solved by technology

Although domestic fluorescent PCR technology can detect the common, H7 and N9 subtypes of avian influenza virus respectively, it is time-consuming and labor-intensive, and the procedure is complicated, which cannot meet the needs of rapid detection.

So far, there is no country that can accurately and quickly identify the pathogenic detection methods of common avian influenza virus, H7, and N9 subtypes in China. In view of the prevalence of the virus in our province, it is necessary to establish a triple fluorescent RT-PCR detection method for the identification of common, H7 and N9 subtypes of avian influenza virus

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