Growth-promoting biological bacterial fertilizer for saline-alkali land
A technology of biological bacterial fertilizer and saline-alkali land, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as inconvenience
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Embodiment 1
[0084] A: Production of complex microbial agents
[0085]a. inoculate Trichoderma aculeatus into PDA culture fluid, and carry out liquid fermentation; the production conditions of said liquid fermentation are: initial pH value 6.7-7.2, culture temperature 25-30 ℃, dissolved oxygen ventilation range 40-95%, 170rpm ;Ferment for 24 hours, the number of bacteria in the fermentation broth is ≥2 billion / ml;
[0086] B. inoculate the Trichoderma aculeatus fermented liquid that step a makes into the solid fermentation substrate, ferment for 6 days and dry at low temperature to obtain the Trichoderma aculeatus solid strain; the inoculum size is 7% by volume; the solid fermentation substrate For, vinegar tank:wheat bran mass ratio 5:1, sterilized at 120°C for 30min;
[0087] c. Inoculate Bacillus subtilis into beef broth peptone liquid fermentation medium for liquid fermentation; the liquid fermentation production conditions are: initial pH value 6.7-7.2, culture temperature 35-37 °C, ...
Embodiment 2
[0097] A: Production of complex microbial agents
[0098] a. inoculate Trichoderma aculeatus into PDA culture fluid, and carry out liquid fermentation; the production conditions of said liquid fermentation are: initial pH value 6.7-7.2, culture temperature 25-30 ℃, dissolved oxygen ventilation range 40-95%, 175rpm ;Ferment for 24 hours, the number of bacteria in the fermentation broth is ≥2 billion / ml;
[0099] B. inoculate the Trichoderma aculeatus fermented liquid that step a makes into the solid fermentation substrate, ferment for 6 days and dry at low temperature to obtain the Trichoderma aculeatus solid strain; the inoculum size is 7% by volume; the solid fermentation substrate For, vinegar tank:wheat bran mass ratio 5:1, sterilized at 120°C for 30min;
[0100] c. Inoculate Bacillus subtilis into beef broth peptone liquid fermentation medium for liquid fermentation; the liquid fermentation production conditions are: initial pH value 6.7-7.2, culture temperature 35-37 °C,...
Embodiment 3
[0110] A: Production of complex microbial agents
[0111] a. Trichoderma aculeatus is inoculated into PDA culture fluid, and liquid fermentation is carried out; the production conditions of said liquid fermentation are: initial pH value 6.7-7.2, culture temperature 25-30 ℃, dissolved oxygen ventilation range 40-95%, 165rpm ;Ferment for 24 hours, the number of bacteria in the fermentation broth is ≥2 billion / ml;
[0112] B. inoculate the Trichoderma aculeatus fermented liquid that step a makes into the solid fermentation substrate, ferment for 6 days and dry at low temperature to obtain the Trichoderma aculeatus solid strain; the inoculum size is 7% by volume; the solid fermentation substrate For, vinegar tank:wheat bran mass ratio 5:1, sterilized at 120°C for 30min;
[0113] c. Inoculate Bacillus subtilis into beef broth peptone liquid fermentation medium for liquid fermentation; the liquid fermentation production conditions are: initial pH value 6.7-7.2, culture temperature ...
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