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A kind of natural concentrated dietary fiber and its preparation method

A dietary fiber, natural technology, applied in the direction of bacteria, food processing, food science, etc. used in food preparation, can solve the problems of high insoluble fiber content, poor feed palatability, low fiber content, etc., to reduce production costs and acid production. The effect of high capacity and low enzyme dosage

Active Publication Date: 2021-05-18
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the commonly used sources of fiber in feed include conventional feed materials such as wheat bran, soybean hulls, sugar beet pulp, and corn bran. Not only are these crude fiber materials low in fiber content (for example, the crude fiber content in bran generally does not exceed 10%) , when the addition amount is high, it will easily lead to poor palatability of the feed; moreover, these raw materials contain high insoluble fiber content, which is difficult to be fermented, resulting in low utilization rate

Method used

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  • A kind of natural concentrated dietary fiber and its preparation method
  • A kind of natural concentrated dietary fiber and its preparation method
  • A kind of natural concentrated dietary fiber and its preparation method

Examples

Experimental program
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Embodiment 1

[0029] Example 1: Isolation and identification of a strain of Lactobacillus plantarum

[0030] 1. Take healthy piglet small intestine (such as duodenum, jejunum, ileum, cecum, colon) intestinal mucosa 5cm 2 , put it in a sterilized beaker, wash the contents on the mucosa with sterile saline, immerse the mucosa in a beaker filled with 15mL of HEPES buffer, shake the mucosa back and forth with tweezers for 5min, and draw 0.2mL of the supernatant with a sterile syringe Put the liquid into the Hungates rolling tube with Rogosa SL agar, roll it gently by hand to make the supernatant evenly distributed on the surface of the agar in the tube, put in 5% CO 2Incubator, after incubating at 37°C for 72 hours, perform aseptic operation in a biological safety cabinet, use a needle to pick up white needle-sized colonies into anaerobic sterile Rogosa broth for cultivation, observe whether the broth becomes turbid, and select well-growing, If there is turbidity, store in a 4°C refrigerator f...

Embodiment 2

[0043] Example 2: Effect of different pine bark raw materials on natural concentrated dietary fiber

[0044] 1) After cleaning and removing impurities from the bark of Yunnan pine, larch and masson pine, crush them through a 20-mesh sieve, add room temperature water to soak, the ratio of crushed raw materials to room temperature water is 1g: 10mL, soaking time is 4h , to discharge the waste water.

[0045] 2) Transfer the three kinds of pine bark raw materials obtained in step 1) to the reactor respectively, add water again, the ratio of pine bark raw material to normal temperature water is 1g:20mL, and carry out ultrasonic-assisted enzymolysis, which is specifically after ultrasonic pretreatment for 5 minutes , adding 1g enzyme activity in every liter of mixed solution is the pectinase of 1.0U / mg (the product number that U.S. Sigma company sells is the pectinase of 17389) and 5g enzyme activity is the papain (the U.S. Sigma company sells) of 12U / mg The product number is 7621...

Embodiment 3

[0051] Example 3: Effects of Fermentation of Different Probiotics on Natural Concentrated Dietary Fiber

[0052] 1) After cleaning and removing impurities from the bark of pine yunnanensis, crush it through a 30-mesh sieve, add room temperature water to soak, the ratio of crushed raw material to room temperature water is 1g:5mL, soak for 2 hours, and drain the waste water.

[0053] 2) Transfer the pine bark raw material obtained in step 1) to the reactor, add water again, the ratio of pine bark raw material to normal temperature water is 1g: 10mL, and carry out ultrasonic-assisted enzymolysis, which is specifically after ultrasonic pretreatment for 10 minutes. Adding 1.2g enzyme activity is the pectinase of 1.0U / mg (the product number sold by U.S. Sigma company is the pectinase of 17389) and 6g enzyme activity is the papain (the product sold by U.S. Sigma company) of 12U / mg No. 76218 papain) for enzymolysis, the enzymolysis temperature is 55°C, the enzymolysis pH value is 6.0,...

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Abstract

A kind of natural concentrated dietary fiber and preparation method thereof, described natural concentrated dietary fiber is that the pine bark that is rich in dietary fiber is crushed and sieved, soaked in water, drains waste water after soaking, and then transfers the soaked pine bark To the reactor, add water to obtain the pine bark mixed liquid, carry out ultrasonic-assisted enzymolysis treatment, drain the liquid after enzymolysis, then insert the enzymolysis material into Lactobacillus plantarum HKB‑38 for fermentation, and ferment the fermented The material is subjected to high temperature and high pressure heat treatment, cooled and dried to obtain natural concentrated dietary fiber. The invention also includes a preparation method of natural concentrated dietary fiber. The invention selects pine bark with low price and abundant resources as the production raw material, greatly reduces the production cost, makes full use of resources and creates high added value. The invention significantly improves the purity of the dietary fiber and the swelling and water holding capacity of the fiber product. Experiments have proved that in the concentrated dietary fiber of the present invention, the crude fiber content is ≥ 60%, the neutral detergent fiber (NDF) content is ≥ 86%, the acid detergent fiber (ADF) content is ≥ 70%, the product swelling property is ≥ 5.0mL / g, and the water holding capacity ≥5.0g / g.

Description

technical field [0001] The invention relates to a dietary fiber and a preparation method thereof, in particular to a natural concentrated dietary fiber and a preparation method thereof. Background technique [0002] Dietary fiber is a general term for polysaccharide carbohydrates and lignin that are not digested and absorbed by the human body. Its physiological functions are mainly the following aspects: 1) Promote gastrointestinal peristalsis and reduce constipation; The intestine is transferred to the anterior intestine, and some components of dietary fiber also have the effect of adsorbing toxins and pathogenic bacteria; 3) Dietary fiber can stabilize blood sugar concentration and reduce blood cholesterol levels; 4) Soluble / fermentable dietary fiber is fermented in the colon , and provide nutrients for the microorganisms in the colon, which is conducive to stabilizing the balance of the intestinal flora; 5) The short-chain fatty acids (acetic acid, propionic acid and buty...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04A23K10/12A23K10/14A23K10/37A23K20/163A23K20/111
CPCA23K10/12A23K10/14A23K10/37A23K20/111A23K20/163A23V2400/169Y02P60/87
Inventor 贺建华伍小松刘明杨凤娟董彦君
Owner HUNAN AGRICULTURAL UNIV
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