Novel composite microecological preparation specially used for piglets and preparation method thereof
A technology of compound microecology and preparation, applied in application, animal husbandry, animal feed, etc., can solve problems affecting the quality of livestock and poultry products, drug residue environment, health hazards, etc., to improve the ability of anti-bacteria and anti-virus, reduce Diarrhea rate, effect of promoting animal growth and development
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Embodiment 1
[0030] Embodiment 1: the preparation of piglet feed additive
[0031]A. Activated slant strains: use LB medium with a pH of 7-7.5 and sterilized at 120°C for 30 minutes to culture Bacillus subtilis CCTCC No.M207086, Lactobacillus acidophilus CGMCC No.10188 and Saccharomyces cerevisiae CCTCC NO: M207178 , cultured at 34°C for 16 hours;
[0032] B. Primary liquid expansion culture: Carry out primary liquid expansion culture for Bacillus subtilis CCTCC No.M207086, Lactobacillus acidophilus CGMCC NO.10188 and Saccharomyces cerevisiae CCTCC NO:M207178 respectively, with an inoculum size of 15%;
[0033] C. Secondary liquid expansion culture: carry out secondary liquid expansion culture on Bacillus subtilis CCTCC No.M207086, Lactobacillus acidophilus CGMCC NO.10188 and Saccharomyces cerevisiae CCTCC NO:M207178 after the first-level liquid expansion culture, the inoculation amount is 15% ;
[0034] D. Preparation of compound micro-ecological preparations: Dried the fermentation bro...
Embodiment 2
[0036] Embodiment 2: Rat safety and disease treatment and disease prevention effect test
[0037] The safety of compound probiotics of the present invention and the effect of treating and preventing diseases are explained below in conjunction with test: compound probiotics of the present invention passes rat acute toxicity test in laboratory of Qingdao Agricultural University, and experimental group is 50 rats, and conventional feed is free Diet, feed the feed additive prepared in Example 1 at the same time, and feed it at 0.6g / 10g body weight for 21 days; the blank control group was 50 rats, which were fed free diet according to the routine, and no feed additive was given.
[0038] The results showed that all the rats in the experimental group survived, moved freely, had smooth hair, normal diet, no secretions in the respiratory tract, eyes and mouth, no symptoms of poisoning, and weight gain. Compared with the blank control group, the body weight of the rats in the experimen...
Embodiment 3
[0039] Example 3: Effect test of feed additives on piglet growth performance, morbidity and diarrhea rate
[0040] 200 sows in labor were randomly divided into experimental group and control group according to the expected farrowing date and parity, with 100 pigs in each group. Add 0.5kg piglet feed additive (prepared in embodiment 1) in the per 1000kg diet of the piglets of the test group, evenly mix in the feed and feed; the sows of the control group and the piglets they produce are fed routinely, and the sows are fed on their own. The experiment started after entering the farrowing room and ended at weaning. The experiment was carried out in the same disease-free pig house. During the experiment, feeding management, immunization and disinfection were carried out according to the conventional methods of the pig farm.
[0041] Table 1 Test grouping and feeding composition
[0042]
[0043] Table 2 Effects of feed additives on weight gain of suckling piglets
[0044] ...
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