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Method for grafting sugar oranges through tissue culture seedling stock

A technology of tangerine and tissue culture seedlings, which is applied in grafting, botany equipment and methods, cultivation, etc., can solve the problems of low lignification degree and inability to realize the grafting of citrus buds well, and achieve good root quality, It is beneficial to the healing of grafted wounds and has broad application prospects

Inactive Publication Date: 2018-04-10
ZHONGSHAN JIAYUAN ECOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the grafting of citrus buds, since the citrus buds are new shoots that have germinated recently from citrus plants, the new shoots are not yet mature at this time, and the degree of lignification is very low, so the grafting of citrus buds cannot be well realized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method of using tissue culture seedling rootstock to graft sugar orange, including rootstock selection, scion preparation, seedling grafting, and post-grafting management procedures. The specific operation steps are as follows:

[0029] S1: Rootstock selection: select the young and tender stem segments on the top of the mother plant of the fruit-hanging sugar tangerine as explants. After the stem segments are disinfected, they are cultivated for 3 months according to the conventional tissue culture seedling cultivation method to obtain the sugar tangerine tissue culture seedlings. Then transplant the tissue culture seedlings to a seedling raising cup equipped with a seedling substrate, which is composed of pond mud, coconut bran, silkworm excrement and slow and controlled release fertilizer in a weight ratio of 100:45:18:6. Fertilizer NP 2 O 5 -K 2 The ratio of O is 22-10-10, and it is managed by conventional seedling cultivation methods. After 25 days of cultivation, whe...

Embodiment 2

[0035] A method for grafting sugar orange with rootstock of tissue culture seedlings includes the steps of rootstock selection, scion preparation, seedling grafting, and post-grafting management. The specific operation steps are as follows:

[0036] S1: Rootstock selection: select the young and tender stem segments on the top of the mother plant of the fruit-hanging sugar tangerine as the explants. After the stem segments are disinfected, they are cultivated for 2 and a half months according to the conventional tissue culture seedling cultivation method to obtain the sugar tangerine tissue culture seedlings. Then transplant the tissue-cultured seedlings to a seedling raising cup equipped with a seedling substrate, which is composed of pond mud, coconut bran, silkworm excrement and slow and controlled release fertilizer in a weight ratio of 100:50:15:7. Fertilizer NP 2 O 5 -K 2 The ratio of O is 22-10-10, and it is managed by conventional seedling cultivation methods. After 30 days...

Embodiment 3

[0042] A method for grafting sugar orange with rootstock of tissue culture seedlings includes the steps of rootstock selection, scion preparation, seedling grafting, and post-grafting management. The specific operation steps are as follows:

[0043] S1: Rootstock selection: select the young and tender stem segments on the top of the mother plant of the fruit-hanging sugar tangerine as explants. After the stem segments are disinfected, they will be cultivated for 3 months according to the conventional tissue culture method to obtain the sugar tangerine tissue culture seedlings. Then transplant the tissue cultured seedlings to a seedling raising cup equipped with a seedling substrate, which is composed of pond mud, coconut bran, silkworm excrement and slow and controlled release fertilizer in a weight ratio of 100:60:20:6. Fertilizer NP 2 O 5 -K 2 The ratio of O is 22-10-10, and it is managed by conventional seedling cultivation methods. After 20 days of cultivation, when the sugar ...

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Abstract

The invention discloses a method for grafting sugar oranges through tissue culture seedling stock. The method comprises the steps that sugar orange tissue culture seedlings are selected for cultivating new tips to be adopted as the stock, budling growing through apical dominance is cut off from the top ends of the sugar oranges to be adopted as scions, a grafting method is adopted for grafting totightly combine the scions with the stock, the grafted fruit seedlings are cultivated, and sugar orange grafted seedlings are obtained. The sugar orange tissue culture seedlings are adopted as the stock, the tissue culture seedling root system quality is good, the grafted seedlings are recovered rapidly, growth is rapid, the seedling formation time is effectively shortened, the input cost is lowered, and economic benefits are remarkable; meanwhile, the method has the advantages of being easy to implement, low in cost, high in survival rate, capable of effectively shortening the stock culture time in a conventional grafting method and ensuring the sugar orange good stock characteristics, and good in economic benefit, the survival rate of the grafted seedlings reaches 95% or above, and rapiddevelopment of the sugar orange industry is accelerated.

Description

Technical field [0001] The invention relates to the technical field of fruit seedling propagation, in particular to a method for grafting sugar tangerines with rootstocks of tissue culture seedlings. Background technique [0002] Citrus (Citrus reticulata Blanco) is a genus of the Rutaceae family, also known as citrus. my country is one of the important origins of citrus, with rich citrus resources, a wide variety of fine varieties, and a cultivation history of more than 4,000 years. After long-term cultivation and selection, citrus has become a precious fruit for mankind. More than 30 kinds of human health substances contained in citrus have been separated, among which are: flavonoids, monoterpenes, coumarins, carotenoids, propanols, acridinones, glyceroglycolipids, etc. Citrus fruits are edible, and orange peel, citrus leaves, and citrus roots can all be used as medicine. They have different functions such as reducing phlegm, relieving cough, soothing the liver, strengthening...

Claims

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Application Information

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IPC IPC(8): A01G2/30A01G17/00A01G24/10A01G24/25A01G24/20
CPCA01G17/005
Inventor 胡穗林波
Owner ZHONGSHAN JIAYUAN ECOLOGICAL AGRI CO LTD
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