Preparation method and application of monodisperse polymer fluorescent microsphere

A technology of fluorescent microspheres and polymers, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of cumbersome preparation methods and so on

Active Publication Date: 2018-04-06
上海艾瑞德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there are few reports on the preparation of fluorescent microspheres with stable fluorescence performance, controllable size, uniform particle size and high content of surface functional groups, and most of the preparation methods are cumbersome.

Method used

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  • Preparation method and application of monodisperse polymer fluorescent microsphere
  • Preparation method and application of monodisperse polymer fluorescent microsphere
  • Preparation method and application of monodisperse polymer fluorescent microsphere

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preparation example Construction

[0038] (1) Preparation of the oil phase mixture: adding functional reactive monomers, fluorescent molecules, and reactive monomer styrene into the superhydrophobic agent n-hexadecane, and ultrasonically mixing to form an oil phase mixture;

[0039] (2) Preparation of the aqueous phase mixture: take a certain amount of surfactant and disperse it in water, with a total volume of 9 mL, and ultrasonically treat it to completely dissolve it, forming an aqueous phase mixture;

[0040] (3) Preparation of initiator aqueous solution: add initiator to 1mL water, shake to dissolve;

[0041] (4) Emulsification process: add the water phase to the oil phase, stir and ultrasonically carry out pre-emulsification, and then process it with a cell pulverizer under a certain power and ice bath conditions for 10 minutes until complete emulsification;

[0042] (5) Transfer the above-mentioned emulsion to a 100mL three-necked flask, add the aqueous initiator solution prepared in (3), stir and mix we...

Embodiment 1

[0054] Effect of Surfactant Addition on the Particle Size and Dispersion of Polymer Microspheres

[0055] (1) Preparation of the oil phase mixture: Weigh 1.8 g of styrene and 0.2 g of acrylic acid and add it to 0.1 g of superhydrophobic agent n-hexadecane, and ultrasonically mix evenly to form an oil phase mixture;

[0056] (2) Preparation of aqueous phase mixture: the surfactant sodium dodecyl sulfate (SDS) of 0.002g (0.1wt%) or 0.01g (0.5wt%) or 0.02g (1wt%) is dispersed in water respectively, The total volume is 9mL, shake to dissolve completely, and it is an aqueous phase mixture;

[0057] (3) Preparation of initiator aqueous solution: Weigh 0.02g of potassium persulfate into 1mL of water, shake to dissolve;

[0058] (4) Emulsification: add the water phase to the oil phase, stir or ultrasonically carry out pre-emulsification, and then process it with a cell pulverizer for 10 minutes under the condition of 250W power and ice bath until complete emulsification;

[0059] (5...

Embodiment 2

[0062] The preparation of the polymer fluorescent microspheres of carboxyl functionalization comprises the following steps:

[0063] (1) Preparation of oil phase mixture: Weigh 1.8g of styrene, 0.2g of acrylic acid and 0.01g (0.5wt%) of naphthalimide fluorescent molecules into 0.1g of superhydrophobic agent n-hexadecane, and ultrasonically mix Uniform, oil phase mixture;

[0064] (2) Preparation of the aqueous phase mixture: take 0.005 g of surfactant sodium lauryl sulfate and disperse it in water with a total volume of 9 mL, shake it to dissolve completely, and form an aqueous phase mixture;

[0065] (3) Preparation of initiator aqueous solution: Weigh 0.02g of potassium persulfate into 1mL of water, shake to dissolve;

[0066] (4) Emulsification process: add the water phase to the oil phase, stir or ultrasonically carry out pre-emulsification, and then process it with a cell pulverizer for 10 minutes under the condition of 250W power and ice bath until complete emulsificati...

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Abstract

The invention discloses a preparation method and application of a monodisperse polymer fluorescent microsphere. The invention provides a method for obtaining a functionalized fluorescent microsphere with a surface containing a carboxyl group or an amino group through a one-step method. The method comprises the steps of (1) providing an oil phase mixture, a water phase mixture and an initiator water solution; (2) fully mixing the water phase mixture and the oil phase mixture, pre-emulsifying and completely emulsifying to obtain an emulsion for standby use; (3) uniformly mixing the emulsion forstandby use and the initiator water solution; carrying out polymerization reaction under nitrogen protection and dark conditions, and purifying after finishing reaction, thus obtaining the polymer fluorescent microsphere. The preparation method provided by the invention is simple, the process is environmentally-friendly, and the cost is low; the obtained fluorescent microsphere is controllable inparticle size, uniform in size, high in fluorescent brightness, high in functional group content, and stable in performance, and has a better application prospect in immune side chromatography.

Description

technical field [0001] The invention relates to the fields of polymer nanocomposite materials and medical detection, in particular to a preparation method of polymer fluorescent microspheres and its application in immunological lateral chromatography. Background technique [0002] Fluorescent microspheres generally refer to microspheres with sizes ranging from nanometers to micrometers, and loaded with fluorescent substances on the surface or inside, which can excite fluorescence under the stimulation of external energy. Due to the stable morphological structure, good fluorescence characteristics and high fluorescence sensitivity of fluorescent microspheres, they have a very wide range of applications in the field of biomedicine. Fluorescence immunochromatography technology uses the active functional groups on the surface of fluorescent microspheres to bind to antibodies, which can realize the detection of the antigen to be tested in the blood. This technology can achieve a...

Claims

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Application Information

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IPC IPC(8): C09K11/06C08F212/08C08F220/06C08J3/12
CPCC08F212/08C08J3/12C08J2325/08C09K11/06C08F220/06
Inventor 操凤文陈凯敏麻玉雯瞿祎袁望董志茂李博学王佩瑞叶一肖琨倪晓涛陆亮朱轩仪杨茜茹李秋萍李欢曹秋岑
Owner 上海艾瑞德生物科技有限公司
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