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Differentiation agent and method for inducing differentiation of mouse preadipocyte 3t3-l1

A technology for 3T3-L1 and preadipocytes, applied in cell culture active agents, biochemical equipment and methods, animal cells, etc., can solve the problems of difficulty in inducing and differentiation of mouse preadipocyte 3T3-L1 and short differentiation cycle

Active Publication Date: 2021-08-20
上海银海圣生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of difficult induction and differentiation of mouse preadipocyte 3T3-L1 in the prior art, and to provide a method for inducing differentiation of mouse preadipocyte 3T3-L1 with short differentiation cycle, high transformation rate and consistent differentiation

Method used

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  • Differentiation agent and method for inducing differentiation of mouse preadipocyte 3t3-l1

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Effect test

Embodiment 1

[0028] The induction and differentiation agents of mouse preadipocyte 3T3-L1 consisted of: 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone, and FBS. The ratio of the above components is: 0.5mmol / L:5μg / mL:1μmol / L:1μmol / L:100mL / L.

Embodiment 2

[0030] The induction and differentiation agents of mouse preadipocyte 3T3-L1 consisted of: 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone, and FBS. The ratio of the above components is: 0.45mmol / L:5.5μg / mL:0.9μmol / L:1.1μmol / L:100mL / L.

Embodiment 3

[0032] The induction and differentiation agents of mouse preadipocyte 3T3-L1 consisted of: 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone, and FBS. The ratio of the above components is: 0.55mmol / L:4.5μg / mL:1.1μmol / L:0.9μmol / L:100mL / L.

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Abstract

The invention provides an agent for inducing differentiation of mouse preadipocyte 3T3‑L1 and a method for inducing differentiation. The differentiation-inducing agent consists of: 3-isobutyl-1-methylxanthine, insulin, dexamethasone, rosiglitazone, and FBS. The method of inducing differentiation is as follows: 3T3-L1 cells are cultured with high-sugar DMEM medium containing the above-mentioned differentiation-inducing agent after routine resuscitation culture, then cultured with high-sugar DMEM medium containing insulin and 10% FBS, and then cultured with high-sugar DMEM medium containing 10% FBS The high-glucose DMEM culture medium was cultured by the half-liquid exchange method. The method of the present invention has a short differentiation period of only 6-7 days, a high conversion rate of preadipocytes, and consistent cell differentiation, which will greatly promote the development of research on fat metabolism diseases.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to an agent for inducing differentiation of mouse preadipocyte 3T3-L1 and a method for inducing differentiation. Background technique [0002] Mouse preadipocyte 3T3-L1 is a cell line isolated and cloned from mouse embryos with single differentiation potential, which can be induced to differentiate into mature adipocytes under certain conditions. The mature adipocytes differentiated from 3T3-L1 can better mimic the fat cells in the body in terms of morphology and function, so 3T3-L1 is an important tool cell for studying metabolic syndromes such as obesity, diabetes, and non-alcoholic fatty liver. [0003] The cell differentiation process of 3T3-L1 is a comprehensive regulation process of gene level and hormone level. However, 3T3-L1 preadipocytes are not only difficult to culture, but also to induce differentiation and maturation has become the bottleneck of many scientific researches....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0653C12N2500/40C12N2501/33C12N2501/385C12N2501/39
Inventor 李欣伦江泉肖泽玺祝加和李超刘媛媛张双李秋圆
Owner 上海银海圣生物科技有限公司
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